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    Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva

    Access Status
    Fulltext not available
    Authors
    Harling, R.
    Kenyon, L.
    Lewis, B.
    Oliver, Richard
    Turner, J.
    Coddington, A.
    Date
    1988
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    HARLING R, KENYON L, LEWIS BG, OLIVER RP, TURNER JG & CODDINGTON A (1988) Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva. Journal of Phytopathology 122 143-146
    DOI
    10.1111/j.1439-0434.1988.tb01001.x
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/12393
    Collection
    • Curtin Research Publications
    Abstract

    A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support.

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