Show simple item record

dc.contributor.authorHarling, R.
dc.contributor.authorKenyon, L.
dc.contributor.authorLewis, B.
dc.contributor.authorOliver, Richard
dc.contributor.authorTurner, J.
dc.contributor.authorCoddington, A.
dc.date.accessioned2017-01-30T11:30:29Z
dc.date.available2017-01-30T11:30:29Z
dc.date.created2010-11-12T04:09:35Z
dc.date.issued1988
dc.identifier.citationHARLING R, KENYON L, LEWIS BG, OLIVER RP, TURNER JG & CODDINGTON A (1988) Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva. Journal of Phytopathology 122 143-146
dc.identifier.urihttp://hdl.handle.net/20.500.11937/12393
dc.identifier.doi10.1111/j.1439-0434.1988.tb01001.x
dc.description.abstract

A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support.

dc.titleConditions for efficient isolation and regeneration of protoplasts from Fulvia fulva
dc.typeJournal Article
curtin.note

A copy of this item may be available from Professor Richard Oliver

curtin.note

Email: Richard.oliver@curtin.edu.au

curtin.accessStatusFulltext not available
curtin.facultyDepartment of Environmental & Agriculture
curtin.facultySchool of Agriculture and Environment
curtin.facultyFaculty of Science and Engineering


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record