The effect of cell culture on X chromosome mosaicism
Access Status
Open access
Authors
Date
2013Supervisor
Dr Ashleigh Murch
Prof. John Wetherall
Dr Narelle Hadlow
Type
Thesis
Award
MSc
Metadata
Show full item recordSchool
School of Biomedical Science
Collection
Abstract
The effects of cell culture on X chromosome aneuploidy in young women with established fertility were investigated. Fluorescence in situ hybridisation was performed on paired samples of mitogen stimulated and uncultured lymphocytes using probes for the X and 9 chromosomes. All participants had <5% X aneuploidy in both preparations. X chromosome loss occurred more frequently than gain. These results provide important information when interpreting the low levels of X mosaicism seen in routine cytogenetic practice.
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