View Item 

The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula

Access Status
Fulltext not available
Authors
Nelson, M.
Phan, H.
Hane, James
Williams, A.
O'Lone, C.
Fosu-Nyarko, J.
Scobie, M.
Cakir, M.
Jones, M.
Bellgard, M.
Ksiazkiewicz, M.
Wolko, B.W.
Barker, S.
Oliver, Richard
Cowling, W.
Date
2006
Type
Journal Article

Metadata
Show full item record
Citation
Nelson, M. and Phan, H. and Hane, J. and Williams, A. and O'Lone, C. and Fosu-Nyarko, J. and Scobie, M. et al. 2006. The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula. Theoretical and Applied Genetics. 113 (2): pp. 225-238.
Source Title
Theoretical and Applied Genetics
DOI
ISSN
URI
Collection
Abstract

We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.