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Aligning a New Reference Genetic Map of Lupinus angustifolius with the Genome Sequence of the Model Legume, Lotus japonicus

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Open access
Authors
Nelson, M.
Moolhuijzen, P.
Boersma, J.
Chudy, M.
Lesniewska, K.
Bellgard, M.
Oliver, Richard
Swiecicki, W.
Wolko, B.W.
Cowling, W.
Ellwood, Simon
Date
2010
Type
Journal Article

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Nelson, M. and Moolhuijzen, P. and Boersma, J. and Chudy, M. and Lesniewska, K. and Bellgard, M. and Oliver, R. et al. 2010. Aligning a New Reference Genetic Map of Lupinus angustifolius with the Genome Sequence of the Model Legume, Lotus japonicus. DNA RESEARCH. 17: pp. 73-83.
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DNA RESEARCH
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Department of Environment and Agriculture
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This is a pre-copy-editing, author-produced PDF of an article accepted for publication in DNA Research following peer review. The definitive publisher-authenticated version Nelson, Matthew N. and Moolhuijzen, Paula M. and Boersma, Jeffrey G. and Chudy, Magdalena and Lesniewska, Karolina and Bellgard, Matthew and Oliver, Richard P. and Swiecicki, Wojciech and Wolko, Bogdan and Cowling, Wallace A. and Ellwood, Simon R. 2010. Aligning a New Reference Genetic Map of Lupinus angustifolius with the Genome Sequence of the Model Legume, Lotus japonicus. DNA Research. 17 (2): pp. 73-83 is available online at: http://dx.doi.org/10.1093/dnares/dsq001

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Abstract

We have developed a dense reference genetic map of Lupinus angustifolius (2n 5 40) based on a set of 106 publicly available recombinant inbred lines derived from a cross between domesticated and wild parental lines. The map comprised 1090 loci in 20 linkage groups and three small clusters, drawing together data from several previous mapping publications plus almost 200 new markers, of which 63 were genebased markers. A total of 171 mainly gene-based, sequence-tagged site loci served as bridging points for comparing the Lu. angustifolius genome with the genome sequence of the model legume, Lotus japonicus via BLASTn homology searching. Comparative analysis indicated that the genomes of Lu. angustifolius and Lo. japonicus are highly diverged structurally but with significant regions of conserved synteny including the region of the Lu. angustifolius genome containing the pod-shatter resistance gene, lentus. We discuss the potential of synteny analysis for identifying candidate genes for domestication traits in Lu. angustifolius and in improving our understanding of Fabaceae genome evolution.