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dc.contributor.authorBrowne, P.
dc.contributor.authorBarret, M.
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorMorrissey, J.
dc.date.accessioned2017-01-30T11:47:23Z
dc.date.available2017-01-30T11:47:23Z
dc.date.created2015-03-03T03:50:50Z
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/20.500.11937/15034
dc.identifier.doi10.1186/1471-2180-10-300
dc.description.abstract

Background: Catabolite repression control (CRC) is an important global control system in Pseudomonas that fine tunes metabolism in order optimise growth and metabolism in a range of different environments. The mechanism of CRC in Pseudomonas spp. centres on the binding of a protein, Crc, to an A-rich motif on the 5' end of an mRNA resulting in translational down-regulation of target genes. Despite the identification of several Crc targets in Pseudomonas spp. the Crc regulon has remained largely unexplored. Results: In order to predict direct targets of Crc, we used a bioinformatics approach based on detection of A-rich motifs near the initiation of translation of all protein-encoding genes in twelve fully sequenced Pseudomonas genomes. As expected, our data predict that genes related to the utilisation of less preferred nutrients, such as some carbohydrates, nitrogen sources and aromatic carbon compounds are targets of Crc. A general trend in this analysis is that the regulation of transporters is conserved across species whereas regulation of specific enzymatic steps or transcriptional activators are often conserved only within a species. Interestingly, some nucleoid associated proteins (NAPs) such as HU and IHF are predicted to be regulated by Crc. This finding indicates a possible role of Crc in indirect control over a subset of genes that depend on the DNA bending properties of NAPs for expression or repression. Finally, some virulence traits such as alginate and rhamnolipid production also appear to be regulated by Crc, which links nutritional status cues with the regulation of virulence traits.Conclusions: Catabolite repression control regulates a broad spectrum of genes in Pseudomonas. Some targets are genus-wide and are typically related to central metabolism, whereas other targets are species-specific, or even unique to particular strains. Further study of these novel targets will enhance our understanding of how Pseudomonas bacteria integrate nutritional status cues with the regulation of traits that are of ecological, industrial and clinical importance.

dc.publisherBioMed Central Ltd.
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003667/
dc.titleComputational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria
dc.typeJournal Article
dcterms.source.volume10
dcterms.source.issn1471-2180
dcterms.source.titleBMC Microbiology
curtin.accessStatusFulltext not available


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