Sarcostemma viminale: a potential anticancer therapy
MetadataShow full item record
There is a need for cancer treatments to be selectively cytotoxic to cancer cells so as to reduce adverse side effects. In this study, a cancer cell line (HeLa cells) and a non-cancer cell line (HF-32) were exposed to an extract from the plant Sarcostemma viminale. Cytopathic effects and apoptosis were measured by morphological changes, annexin V/propidium iodide (PI) and 4′,6-diamidino-2-phenylindole (DAPI) staining assays. Also, a novel mixed culture flow cytometry assay was performed exploiting the overexpression of p16INK4a in HeLa cells to demonstrate the change in numbers of HeLa and HF-32 cells post-exposure to the extract. At 1 % (v/v) after 48 h of exposure, HeLa cells showed >75 % cytopathic effect, 77 % were in apoptosis or dead by the annexinV/PI assay, and 100 % had nuclear changes by DAPI staining; there was a reduction of 76 % in the number of cells by mixed culture assay. In contrast, for the HF-32 cells, only 5 % showed any cytopathic effect, there were no more cells in apoptosis or dead (34 %) than in the control by the annexinV/PI assay, <1 % of cells had nuclear changes by DAPI staining, and there was a slight increase in cell numbers by the mixed culture assay. Results from these assays clearly demonstrate that the extract from S. viminale destroyed the cancer HeLa cells quickly and at a low concentration, whilst the non-cancer HF-32 cells survive. This study indicates that extracts from S. viminale may be a specific anticancer agent.
Showing items related by title, author, creator and subject.
Computational modelling of LY303511 and TRAIL-induced apoptosis suggests dynamic regulation of cFLIPShi, Y.; Mellier, G.; Huang, S.; White, J.; Pervaiz, Shazib; Tucker-Kellogg, L. (2013)Motivation: TRAIL has been widely studied for the ability to kill cancer cells selectively, but its clinical usefulness has been hindered by the development of resistance. Multiple compounds have been identified that ...
Brestovac, Brian; Costantino, Paul; Groth, David; Wong, M. (2014)Infection with oncogenic human papillomavirus (HPV) genotypes is necessary for the development of cervical cancer. Testing for HPV DNA from liquid based cervical samples can be used as an adjunct to traditional cytological ...
Breast cancer stem-like cells are inhibited by diosgenin, a steroidal saponin, by the attenuation of the Wnt ß-catenin signaling via the Wnt antagonist secreted frizzled related protein-4Bhuvanalakshmi, G.; Basappa; Rangappa, K.; Dharmarajan, Arunasalam; Sethi, G.; Kumar, Alan Prem; Warrier, Sudha (2017)© 2017 Bhuvanalakshmi, Basappa, Rangappa, Dharmarajan, Sethi, Kumar and Warrier.Background: Identification of breast cancer stem cells as the chemo-resistant and tumor-initiating population represents an important milestone ...