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    A rapid DNA extraction method suitable for human papillomavirus detection

    195527_195527.pdf (150.4Kb)
    Access Status
    Open access
    Authors
    Brestovac, Brian
    Costantino, Paul
    Groth, David
    Wong, M.
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Brestovac, Brian and Wong, Michelle and Costantino, Paul S. and Groth, David. 2014. A rapid DNA extraction method suitable for human papillomavirus detection. Journal of Medical Virology. 86 (4): pp. 653-657.
    Source Title
    Journal of Medical Virology
    DOI
    10.1002/jmv.23882
    ISSN
    10969071
    Remarks

    This is the accepted version of the following article: Brestovac, Brian and Wong, Michelle and Costantino, Paul S. and Groth, David. 2014. A rapid DNA extraction method suitable for human papillomavirus detection. Journal of Medical Virology. 86 (4): pp. 653-657, which has been published in final form at http://doi.org/10.1002/jmv.23882

    URI
    http://hdl.handle.net/20.500.11937/26805
    Collection
    • Curtin Research Publications
    Abstract

    Infection with oncogenic human papillomavirus (HPV) genotypes is necessary for the development of cervical cancer. Testing for HPV DNA from liquid based cervical samples can be used as an adjunct to traditional cytological screening. In addition there are ongoing viral load, genotyping, and prevalence studies. Therefore, a sensitive DNA extraction method is needed to maximize the efficiency of HPV DNA detection. The XytXtract Tissue kit is a DNA extraction kit that is rapid and so could be useful for HPV testing, particularly in screening protocols. This study was undertaken to determine the suitability of this method for HPV detection. DNA extraction from HeLa and Caski cell lines containing HPV 18 and 16 respectively together with DNA from five liquid based cervical samples were used in a HPV PCR assay. DNA was also extracted using the QIAamp DNA mini kit (Qiagen, Hilden, Germany) as a comparison. DNA extracts were serially diluted and assayed. HPV DNA was successfully detected in cell lines and cervical samples using the XytXtract Tissue kit. In addition, the XytXtract method was found to be more sensitive than the QIAmp method as determined by a dilution series of the extracted DNA. While the XytXtract method is a closed, the QIAamp method uses a spin column with possible loss of DNA through DNA binding competition of the matrix, which could impact on the final extraction efficiency. The XytXtract is a cheap, rapid and efficient method for extracting HPV DNA from both cell lines and liquid based cervical samples.

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