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dc.contributor.authorWang, J.
dc.contributor.authorYang, H.
dc.contributor.authorZuo, Zhili
dc.contributor.authorYan, X.
dc.contributor.authorWang, Y.
dc.contributor.authorLuo, X.
dc.contributor.authorJiang, H.
dc.contributor.authorChen, K.
dc.contributor.authorZhu, W.
dc.date.accessioned2017-01-30T12:04:13Z
dc.date.available2017-01-30T12:04:13Z
dc.date.created2011-03-16T20:01:52Z
dc.date.issued2010
dc.identifier.citationWang, Jinan and Yang, Huaiyu and Zuo, Zhili and Yan, Xiuhua and Wang, Yong and Luo, Xiaomin and Jiang, Hualiang and Chen, Kaixian and Zhu, Weiliang. 2010. Molecular Dynamics Simulations on the Mechanism of Transporting Methylamine and Ammonia by Ammonium Transporter AmtB. Journal of Physical Chemistry B. 114 (46): pp. 15172-15179.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/17824
dc.identifier.doi10.1021/jp104508k
dc.description.abstract

AmtB is one of the ammonium transporter proteins facilitating the ammonium transport across the cellular membranes. Experimentally, the substrate used in in vitro studies is the radio labeled [14C]methylammonium, rather than ammonium itself. To explore the similarity and difference of the conduction mechanism of methylamine and ammonia molecules through AmtB, molecular dynamics simulations on 22 carefully designed systems were performed, which demonstrated that methylamine could be automatically transported in a very similar way to ammonia. The driving force for the conduction is mainly the hydrogen bond network comprising His168, His318, and Tyr32, working in coordination with NH−π interaction with residue Trp212. Then, Ser263 translocated the substrates from the exit gate into the cytoplasm by hydrogen bond interaction.The aromatic ring of Trp212 acted like a springboard to facilitate the translocation of the substrates from site Am2 to Am4 via NH−π interaction. Without the mediation of Trp212, further movement of substrate in the channel would be hampered by the strong hydrogen bonding from His168. In agreement with experimental results, the substrates could be transported by W212F mutant but not by W212A within the simulation time as long as 20 ns. In addition, we predicted that the mutants S263D and S263C remain the function of the transporter but S263A does not. The difference of transporting the two substrates is that methylamine involves more hydrophobic interactions than ammonia. In conclusion, methylamine molecule is a good mimic for investigating the translocation mechanism of ammonium transporter AmtB.

dc.publisherAmerican Chemical Society
dc.titleMolecular Dynamics Simulations on the Mechanism of Transporting Methylamine and Ammonia by Ammonium Transporter AmtB
dc.typeJournal Article
dcterms.source.volume114
dcterms.source.number46
dcterms.source.startPage15172
dcterms.source.endPage15179
dcterms.source.issn10895647
dcterms.source.titleJournal of Physical Chemistry B
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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