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    Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions

    Access Status
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    Authors
    Lareu, Ricky R.
    Zeugolis, D.
    Abu-Rub, M.
    Pandit, A.
    Raghunath, M.
    Date
    2010
    Type
    Journal Article
    
    Metadata
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    Citation
    Lareu, Ricky R. and Zeugolis, Dimitrios I. and Abu-Rub, Mohammad and Pandit, Abhay and Raghunath, Michael. 2010. Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions. Acta Biomaterialia. 6 (8): pp. 3146-3151.
    Source Title
    Acta Biomaterialia
    DOI
    10.1016/j.actbio.2010.02.004
    ISSN
    1742-7061
    URI
    http://hdl.handle.net/20.500.11937/18496
    Collection
    • Curtin Research Publications
    Abstract

    Collagen contains the unique imino acid hydroxyproline (HyPro), which is involved in the stabilization of this triple helical molecule. The concentration of HyPro is customarily used to calculate the total collagen content in a cell culture environment and in acid hydrolysates of normal and pathophysiological tissues. Radiolabelling, chromatographic and calorimetric assays have been developed over the years for the accurate determination of collagen content through HyPro estimation. Recently, the Sircol Collagen Assay (SCA) has been almost exclusively adopted as the fastest and simplest colorimetric method for the determination of collagen concentration in complex protein solutions. We show here that the colorimetric SCA, which is based on the binding of Sirius red (SR) to collagen, is flawed by interference of non-collagenous proteins (e.g. serum). In fact, we demonstrate that SCA in cell culture systems and tissue hydrolysates results in a dramatic overestimation of collagen content ranging from 3- to 24-fold. In order to rescue this otherwise very practical assay, we introduce a simple purification procedure that allows the removal of interfering non-collagenous proteins from culture media and tissue samples so that accurate measurements with SCA are now possible.

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