Production of Lactobacilli Proteinases for the Manufacture of Bioactive Peptides: Part II-Downstream Processes
MetadataShow full item record
This chapter deals with the downstream processes of lactobacilli proteinases recovery, isolation and purification strategies. One of the most pressing bioprocess challenges in the area of production of pharmaceutical/biological materials is that of isolation and purification of the finished products. Although several isolation and purification methods exist for proteins, they must all be weighed out based on their merits and demerits. From the already established molecular and particle separations using membranes, centrifugation and phase-partitioning techniques, combinatoric techniques can be developed to ensure proteinase purification at the minimum allowed cost and high product purity. The industrial-scale use and commercialisation of lactobacilli proteinases will require the inputs of scientists, engineers, controls specialists, quality experts and economists. Further, with the important role and prospect of lactobacilli proteinases in industry, more targeted research is needed to aid in the development, purification and characterisation of robust enzymes with desired properties for wider industrial purposes.
Showing items related by title, author, creator and subject.
Production of Lactobacilli Proteinases for the Manufacture of Bioactive Peptides: Part I-Upstream ProcessesAgyei, D.; Potumarthi, R.; Danquah, Michael (2013)This chapter focuses on the metabolism of genus Lactobacillus, with emphasis on the proteolytic potential of the well-known species in order to highlight their potential use in the production of bioactive peptides. ...
Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extractionAgyei, D.; Lim, W.; Zass, M.; Tan, D.; Danquah, Michael (2013)Lactobacilli cell-envelope proteinases (CEPs) have demonstrated numerous biopharmaceutical applications in the development of new streams of blockbuster nutraceuticals; thus, the development of efficient and commercially ...
Carbohydrate utilization affects Lactobacillus delbrueckii subsp. lactis 313 cell-enveloped-associated proteinase productionAgyei, D.; Danquah, Michael (2012)The effect of different sugars (glucose, glycerol, maltose, galactose and lactose) on cell-membrane-associated proteinase production by Lactobacillus delbrueckii subsp. lactis 313 (LDL 313) was investigated. The experimental ...