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Isolation and characterization of EST-derived microsatellite loci from the fungal wheat pathogen Phaeosphaeria nodorum

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Authors
Stukenbrock, E.
Banke, S.
Zala, M.
McDonald, B.
Oliver, Richard
Date
2005
Type
Journal Article

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STUKENBROCK EH, BANKE S, ZALA M, MCDONALD BA, OLIVER RP (2005) Isolation and characterization of EST-derived microsatellite loci from the fungal wheat pathogen Phaeosphaeria nodorum. Molecular Ecology Notes 4 931-933
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Faculty
Department of Environmental & Agriculture
School of Agriculture and Environment
Faculty of Science and Engineering
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A copy of this item may be available from Professor Richard Oliver

Email: Richard.oliver@curtin.edu.au

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Abstract

Eleven polymorphic microsatellite loci and one minisatellite locus originating from expressed sequence tag (EST) libraries of Phaeosphaeria (syn. Stagonospora) nodorum were isolated and characterized. The satellite markers were used to genotype isolates from field populations collected in China, North America and South Africa. The number of alleles per locus ranged from two to 15. Genotype diversity ranged from 87.5 to 95.3 and gene diversity from 0.1 to 0.8. The variable levels of polymorphism within and among populations of P. nodorum renders these 12 satellite loci ideal markers for population genetic analysis of P. nodorum.