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A proteomic analysis of the functional effects of fatty acids in NIH 3T3 fibroblasts

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Open access
Authors
Magdalon, J.
Hatanaka, E.
Romanatto, T.
Rodrigues, H.
Kuwabara, W.
Scaife, C.
Newsholme, Philip
Curi, R.
Date
2011
Type
Journal Article

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Magdalon, Juliana and Hatanaka, Elaine and Romanatto, Talita and Rodrigues, Hosana G. and Kuwabara, Wilson M. T. and Scaife, Caitriona and Newsholme, Philip and Curi, Rui. 2011. A proteomic analysis of the functional effects of fatty acids in NIH 3T3 fibroblasts. Lipids in Health & Disease. 10:218.
Source Title
Lipids in Health & Disease
DOI
School
School of Biomedical Sciences
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This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.

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Abstract

Previous studies have demonstrated that long chain fatty acids influence fibroblast function at sub-lethal concentrations. This study is the first to assess the effects of oleic, linoleic or palmitic acids on protein expression of fibroblasts, as determined by standard proteomic techniques. The fatty acids were not cytotoxic at the concentration used in this work as assessed by membrane integrity, DNA fragmentation and the MTT assay but significantly increased cell proliferation. Subsequently, a proteomic analysis was performed using two dimensional difference gel electrophoresis (2D-DIGE) and MS based identification. Cells treated with 50 μM oleic, linoleic or palmitic acid for 24 h were associated with 24, 22, 16 spots differentially expressed, respectively. Among the identified proteins, α-enolase and far upstream element binding protein 1 (FBP-1) are of importance due to their function in fibroblast-associated diseases. However, modulation of α-enolase and FBP-1 expression by fatty acids was not validated by the Western blot technique.