View Item 

Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

Access Status
Open access
Authors
Hatanaka, Elaine
Dermargos, Alexandre
Hirata, Aparecida
Vinolo, Marco
Carpinelli, Angelo
Newsholme, Philip
Armelin, Hugo
Curi, Rui
Date
2013
Type
Journal Article

Metadata
Show full item record
Citation
Hatanaka, Elaine and Dermargos, Alexandre and Hirata, Aparecida and Vinolo, Marco and Carpinelli, Angelo and Newsholme, Philip and Armelin, Hugo and Curi, Rui. 2013. Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation. PLoS ONE. 8 (4): e58626.
Source Title
PLoS ONE
DOI
ISSN
Remarks

This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.

URI
Collection
Abstract

The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.