Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia
MetadataShow full item record
Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx1a, stx2a, stx2c, and ehxA and the other carrying stx1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.
This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/3.0/
Showing items related by title, author, creator and subject.
Howden, B.; Holt, K.; Lam, M.; Seeman, T.; Ballard, S.; Coombs, Geoffrey; Tong, S.; Grayson, M.; Johnson, P.; Stinear, T. (2013)Nosocomial outbreaks of vancomycin-resistant Enterococcus faecium (VREfm) are thought to occur by transmission of VREfm between patients, predicting that infection control interventions will limit cross-transmission. ...
Low Vancomycin MICs and Fecal Densities Reduce the Sensitivity of Screening Methods for Vancomycin Resistance in EnterococciWijesuriya, T.; Perry, P.; Pryce, T.; Boehm, J.; Kay, I.; Flexman, J.; Coombs, Geoffrey; Ingram, P. (2014)Active surveillance is part of a multifaceted approach used to prevent the spread of vancomycin-resistant enterococci (VRE). The impact of fecal density, the vancomycin MIC of the isolate, and the vancomycin concentration ...
Lim, Tien Tze (2007)Seventy-four methicillin-resistant Staphylococcus aureus (MRSA) from two Malaysian hospitals were characterised by both phenotypic and genotypic techniques. These isolates were collected over an 18 year time period in the ...