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dc.contributor.authorPerera, A.
dc.contributor.authorClarke, C.
dc.contributor.authorDykes, Gary
dc.contributor.authorFegan, N.
dc.date.accessioned2017-01-30T12:22:01Z
dc.date.available2017-01-30T12:22:01Z
dc.date.created2016-01-18T20:00:38Z
dc.date.issued2015
dc.identifier.citationPerera, A. and Clarke, C. and Dykes, G. and Fegan, N. 2015. Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia. BioMed Research International. 2015: Article ID 382403.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/20917
dc.identifier.doi10.1155/2015/382403
dc.description.abstract

Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx2c,  eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx1a, stx2a, stx2c, and ehxA and the other carrying stx1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.

dc.titleCharacterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia
dc.typeJournal Article
dcterms.source.volume2015
dcterms.source.issn2314-6133
dcterms.source.titleBioMed Research International
curtin.note

This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/3.0/

curtin.departmentSchool of Public Health
curtin.accessStatusOpen access


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