Histology of the healing tympanic membrane following perforation in rats
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Objectives/Hypothesis: The aim of this study was to provide a detailed cytological account on the healing tympanic membrane (TM) over 14 days and to complement existing research into TM wound healing. Study Design: The study is a prospective cohort study of 19 male Sprague-Dawley (Rattus norvegicus) rats. Methods: Rat TMs were perforated using a sterile needle and sacrificed at time points during the 14 days following perforation. Results: The healing of the TM resembles cutaneous wound healing except that the TM is unique in the lack of a supportive matrix beneath the regenerating epithelia. This prevents the influx of reparative cells and nutrients and the in growth of the usual fibroblastic reaction. Conclusions: TM wound healing contrasts with cutaneous wound healing in that keratinocytes are the first cells to close the wound and not the last. A keratin scaffold may not be important in the healing process. The malleus plays a crucial role in the healing of the TM and is the site of significant mitotic activity during the healing process. Migration across layers of the TM appears to account for the closure of the perforation. © 2010 The American Laryngological.
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Keratinocyte growth factor 1, fibroblast growth factor 2 and 10 in the healing tympanic membrane following perforation in ratsSanta Maria, P.; Redmond, S.; Atlas, M.; Ghassemifar, Reza (2011)The aim of this study was to provide a transcriptome profile of Keratinocyte Growth Factor (KGF)-1, Fibroblast Growth Factor (FGF) 2 and FGF10 (KGF2) in the healing rat tympanic membrane (TM) over 7 days and an ...
Santa Maria, P.; Redmond, S.; McInnes, R.; Atlas, M.; Ghassemifar, Reza (2011)Objectives/Hypothesis: The aim of this study is to elucidate transcriptional changes that occur in response to tympanic membrane (TM) perforation in rats and to infer key genes and molecular events in the healing process. ...
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