Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    The role of epidermal growth factor in the healing tympanic membrane following perforation in rats

    Access Status
    Fulltext not available
    Authors
    Santa Maria, P.
    Redmond, S.
    Atlas, M.
    Ghassemifar, Reza
    Date
    2010
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Santa Maria, P. and Redmond, S. and Atlas, M. and Ghassemifar, R. 2010. The role of epidermal growth factor in the healing tympanic membrane following perforation in rats. Journal of Molecular Histology. 41 (6): pp. 309-314.
    Source Title
    Journal of Molecular Histology
    DOI
    10.1007/s10735-010-9287-1
    ISSN
    1567-2379
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/5284
    Collection
    • Curtin Research Publications
    Abstract

    Epidermal Growth Factor (EGF) has been identified as playing a critical role in the wound healing process. The objective of this study is to investigate the role that EGF plays in rat tympanic membrane (TM) wound healing using two techniques, microarray and immunohistochemistry. The tympanic membranes of rats were perforated using a sterile needle and sacrificed at time points during 2 weeks following perforation. The normalized signal intensities at the time points for EGF and associated genes are presented. The rat EGF mRNA did not change significantly between time points. Five associated proteins, including heparin-binding EGF-like growth factor were found to be differentially expressed above a two fold threshold at 12 h following perforation. EGF staining was found at low levels in the uninjured TM. Levels of EGF staining increased at 24 h in the basal keratinocyte layer, became diffusely elevated in the specimen at 36 h, before a second peak in staining of the keratinocyte layer at Day 4. The staining of EGF corresponds to its multiple roles in TM wound healing. © 2010 Springer Science+Business Media B.V.

    Related items

    Showing items related by title, author, creator and subject.

    • Histology of the healing tympanic membrane following perforation in rats
      Maria, P.; Redmond, S.; Atlas, M.; Ghassemifar, Reza (2010)
      Objectives/Hypothesis: The aim of this study was to provide a detailed cytological account on the healing tympanic membrane (TM) over 14 days and to complement existing research into TM wound healing. Study Design: The ...
    • Keratinocyte growth factor 1, fibroblast growth factor 2 and 10 in the healing tympanic membrane following perforation in rats
      Santa Maria, P.; Redmond, S.; Atlas, M.; Ghassemifar, Reza (2011)
      The aim of this study was to provide a transcriptome profile of Keratinocyte Growth Factor (KGF)-1, Fibroblast Growth Factor (FGF) 2 and FGF10 (KGF2) in the healing rat tympanic membrane (TM) over 7 days and an ...
    • Tympanic membrane wound healing in rats assessed by transcriptome profiling
      Santa Maria, P.; Redmond, S.; McInnes, R.; Atlas, M.; Ghassemifar, Reza (2011)
      Objectives/Hypothesis: The aim of this study is to elucidate transcriptional changes that occur in response to tympanic membrane (TM) perforation in rats and to infer key genes and molecular events in the healing process. ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.