Electrochemical behaviour of denatured haemoglobin at the liquid|liquid interface
MetadataShow full item record
The link to the journal’s home page is:http://www.elsevier.com/wps/find/journaldescription.cws_home/601449/description#description Copyright © 2010 Elsevier B.V. All rights reserved
We report here the influence of chemical denaturation of haemoglobin on its electrochemical behaviour at the polarised liquid|liquid interface. Denaturation with urea resulted in a modification of the haemoglobin electrochemical behaviour, with the disappearance of the forward transfer peak and a decrease of the reverse peak current. Although the reverse peak current increased linearly with the concentration of denatured haemoglobin in the aqueous phase, the slope of the current-concentration plot was three times lower than that for native haemoglobin over the 0.1-1 μM concentration range. These results indicate the sensitivity of electrochemistry at liquid|liquid interfaces to protein tertiary structure.
Showing items related by title, author, creator and subject.
Herzog, G.; Nolan, M.; Arrigan, Damien (2011)The electrochemical behaviour of haemoglobin denatured using different concentrations of urea was investigated at the liquid|liquid interface. The reverse peak current varied with the concentration of urea, allowing the ...
Interaction of surface-attached haemoglobin with hydrophobic anions monitored by on-line acoustic wave detectorEllis, J.; Xu, S.; Wang, X.; Herzog, G.; Arrigan, Damien; Thompson, M. (2010)The behaviour of proteins on surfaces and at interfaces is an important field with applications in drugdevelopment, clinical diagnostics and studies of device biocompatibility. A key factor is the conformation ofsurface-bound ...
Herzog, G.; KAM, V.; Arrigan, Damien (2008)The electrochemical behaviour of haemoglobin (Hb) at the liquid/liquid interface was investigated. Basedon ion transfer cyclic voltammetry and bulk ionolysis experiments, it is proposed that Hb adsorbs at theliquid/liquid ...