Electrochemical behaviour of denatured haemoglobin at the liquid|liquid interface

Herzog, G.; Eichelmann-Daly, P.; Arrigan, Damien

doi:10.1016/j.elecom.2009.12.020

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Access Status

Open access

Authors

Herzog, G.

Eichelmann-Daly, P.

Arrigan, Damien

Date

2010

Type

Journal Article

Metadata

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Citation

Herzog, Gregoire and Eichelmann-Daly, Patrycja and Arrigan, Damien. 2010. Electrochemical behaviour of denatured haemoglobin at the liquid|liquid interface. Electrochemistry Communications. 12 (3): pp. 335-337.

Source Title

Electrochemistry Communications

DOI

10.1016/j.elecom.2009.12.020

ISSN

13882481

Faculty

Nanochemistry Research Institute (NRI)

Faculty of Science and Engineering

School

Nanochemistry Research Institute (Research Institute)

Remarks

URI

http://hdl.handle.net/20.500.11937/21677

Collection

Curtin Research Publications

Abstract

We report here the influence of chemical denaturation of haemoglobin on its electrochemical behaviour at the polarised liquid|liquid interface. Denaturation with urea resulted in a modification of the haemoglobin electrochemical behaviour, with the disappearance of the forward transfer peak and a decrease of the reverse peak current. Although the reverse peak current increased linearly with the concentration of denatured haemoglobin in the aqueous phase, the slope of the current-concentration plot was three times lower than that for native haemoglobin over the 0.1-1 μM concentration range. These results indicate the sensitivity of electrochemistry at liquid|liquid interfaces to protein tertiary structure.