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    The Determination of Psilocin and Psilocybin in Hallucinogenic Mushrooms by HPLC Utilizing a Dual Reagent Acidic Potassium Permanganate and Tris(2,2'-bipyridyl)ruthenium(II) Chemiluminescence Detection System

    Access Status
    Fulltext not available
    Authors
    Anastos, N.
    Lewis, Simon
    Barnett, N.
    Sims, D.
    Date
    2006
    Type
    Journal Article
    
    Metadata
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    Citation
    Anastos, Nicole and Lewis, Simon W. and Barnett, Neil W. and Sims, D Noel. 2006. The Determination of Psilocin and Psilocybin in Hallucinogenic Mushrooms by HPLC Utilizing a Dual Reagent Acidic Potassium Permanganate and Tris(2,2'-bipyridyl)ruthenium(II) Chemiluminescence Detection System. Journal of Forensic Sciences 51 (1): 45-51.
    Source Title
    Journal of Forensic Sciences
    DOI
    10.1111/j.1556-4029.2005.00033.x
    Faculty
    Department of Applied Chemistry
    Division of Engineering, Science and Computing
    Faculty of Science
    URI
    http://hdl.handle.net/20.500.11937/22010
    Collection
    • Curtin Research Publications
    Abstract

    This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol: 10mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,20-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 x 10-8 and 3.5 x 10-9 mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom.

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