CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages
MetadataShow full item record
In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity. © 2009 Elsevier Inc. All rights reserved.
Showing items related by title, author, creator and subject.
Jackaman, Connie; Tomay, Federica; Duong, Lelinh; Abdol Razak, Norbaini Bintu; Pixley, F.; Metharom, Pat; Nelson, Delia (2017)Impaired immune function has been implicated in the declining health and higher incidence of cancer in the elderly. However, age-related changes to immunity are not completely understood. Neutrophils and macrophages ...
Curi, R.; Mendes, R.; Crispin, L.; Norata, G.; Sampaio, S.; Newsholme, Philip (2017)© 2017 The Author(s). In 1986 and 1987, Philip Newsholme et al. reported macrophages utilize glutamine, as well as glucose, at high rates. These authors measured key enzyme activities and consumption and production levels ...
Phosphatidylinostitol-3 kinase and phospholipase C enhance CSF-1-dependent macrophage survival by controlling glucose uptakeChang, M.; Hamilton, J.; Scholz, G.; Masendycz, P.; Macaulay, S.; Elsegood, Caryn (2009)Colony stimulating factor-1 (CSF-1)-dependent macrophages play crucial roles in the development and progression of several pathological conditions including atherosclerosis and breast cancer metastasis. Macrophages in ...