Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite

    Access Status
    Fulltext not available
    Authors
    Zhou, L.
    Huang, J.
    Yu, B.
    Liu, Yang
    You, T.
    Date
    2015
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Zhou, L. and Huang, J. and Yu, B. and Liu, Y. and You, T. 2015. A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite. ACS Applied Materials and Interfaces. 7 (44): pp. 24438-24445.
    Source Title
    ACS Applied Materials and Interfaces
    DOI
    10.1021/acsami.5b08154
    ISSN
    1944-8244
    School
    Nanochemistry Research Institute
    URI
    http://hdl.handle.net/20.500.11937/23168
    Collection
    • Curtin Research Publications
    Abstract

    © 2015 American Chemical Society. Ru(bpy)3 2+-doped silica (Ru-SiO2) nanoparticles and gold-nanoparticle-decorated graphene (P-RGO@Au) were combined to form a P-RGO@Au@Ru-SiO2 composite. The composite was used to develop a novel sandwich-type electrochemiluminescence immunosensor for the analysis of HIV-1 p24 antigen. The composite worked as carrier to immobilize target antibody and to build a sandwich-type electrochemiluminescence immunosensor through an interaction between antigen and antibody. Importantly, high ECL signal could be obtained due to the large amounts of Ru(bpy)3 2+ molecules per Ru-SiO2 nanoparticle. The P-RGO@Au composite with good conductivity and high surface area not only accelerated the electron transfer rate but also improved the loading of both ECL molecules and capture antibody, which could further increase the ECL response and result in high sensitivity. Taking advantage of both Ru-SiO2 nanoparticles and the P-RGO@Au composite, the proposed immunosensor exhibited a linear range from 1.0 × 10-9 to 1.0 × 10-5 mg mL-1 with a detection limit of 1.0 × 10-9 mg mL-1 for HIV-1 p24 antigen. The proposed ECL immunosensor was used to analyze HIV-1 p24 antigen in human serum, and satisfactory recoveries were obtained, indicating that the proposed method is promising for practical applications in the clinical diagnosis of HIV infection.

    Related items

    Showing items related by title, author, creator and subject.

    • Reduction-Responsive Codelivery System Based on a Metal-Organic Framework for Eliciting Potent Cellular Immune Response
      Yang, Y.; Chen, Q.; Wu, Jian-Ping; Kirk, Brett; Xu, J.; Liu, Z.; Xue, W. (2018)
      © 2018 American Chemical Society. Utilizing nanoparticles to deliver subunit vaccines can be viewed as a promising strategy for enhancing the immune response, especially with regard to cellular immunity to fight against ...
    • Synthesis of polymeric nanocomposite membranes for aqueous and non-aqueous media
      Rajaeian, Babak (2012)
      Thin film composite (TFC) membranes have long been used by many large-scale applications (i.e., water and wastewater treatment). Recently, conventional polymeric TFC membranes are facing with short longevity due to high ...
    • Formulation of chitosan-based nanoparticles for delivery of proteins and peptides
      Vellore Janarthanan, Mohanraj (2003)
      Delivery of complex molecules such as peptides, proteins, oligonucleotides and plasmids is an intensively studied subject, which has attracted considerable medical and pharmaceutical interest. Encapsulation of these ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.