PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene
Access Status
Authors
Date
2010Type
Metadata
Show full item recordCitation
Source Title
ISBN
School
Collection
Abstract
Methicillin resistance, in particular hetero-methicillin resistance, in S aureus and the coagulase negative staphylococci (CNS) can be difficult to detect by phenotypic methods. Subsequently detection of the mecA gene for determining methicillin resistance in staphylococci is generally considered to be the Gold Standard. In Australian diagnostic microbiology laboratories, molecular assays used for the detection of the mecA gene have generally been designed in-house. These assays have either been single primer PCR assays targeting the mecA gene or a duplex primer PCR assay with primers targeting the mecA gene and a gene specific for S. aureus such as the nuc (nuclease) or fem gene. In some laboratories a Panton Valentine-leukocidin (PVL) gene primer has been incorporated with the duplex PCR assay allowing the detection of PVL virulence genes in S aureus. © 2010 Springer Science+Business Media B.V.
Showing items related by title, author, creator and subject.
-
Ito, T.; Hiramatsu, K.; Tomasz, A.; de Lencastre, H.; Perreten, V.; Holden, M.; Coleman, D.; Goering, R.; Giffard, P.; Skov, R.; Zhang, K.; Westh, H.; O'Brien, Frances; Tenover, F.; Oliveira, D.; Boyle-Vavra, S.; Laurent, F.; Kearns, A.; Kreiswirth, B.; Kwan, S.; Grundmann, H.; Sollid, J.; John, J.; Daum, R.; Soderquist, B.; Buist, G. (2012)Methicillin-resistant staphylococci are disseminated all over the world and are frequent causes of health care- and community-associated infections. Methicillin-resistant strains typically carry the acquired mecA gene ...
-
Monecke, S.; Kanig, H.; Rudolph, W.; Müller, E.; Coombs, Geoffrey; Hotzel, H.; Slickers, P.; Ehricht, R. (2010)Background: Community-acquired methicillin-resistant Staphylococcus aureus have become a major problem in Australia. These strains have now been isolated throughout Australia including remote Indigenous communities that ...
-
Worthing, K.; Schwendener, S.; Perreten, V.; Saputra, S.; Coombs, Geoffrey; Pang, S.; Davies, M.; Abraham, S.; Trott, D.; Norris, J. (2018)We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. ...