Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Sss1p is required to complete protein translocon activation

    Access Status
    Open access via publisher
    Authors
    Wilkinson, B.
    Brownsword, J.
    Mousley, Carl
    Stirling, C.
    Date
    2010
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Wilkinson, B. and Brownsword, J. and Mousley, C. and Stirling, C. 2010. Sss1p is required to complete protein translocon activation. Journal of Biological Chemistry. 285 (42): pp. 32671-32677.
    Source Title
    Journal of Biological Chemistry
    DOI
    10.1074/jbc.M110.128256
    ISSN
    0021-9258
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/23985
    Collection
    • Curtin Research Publications
    Abstract

    Protein translocation across the endoplasmic reticulummembrane occurs at the Sec61 translocon. This has two essential subunits, the channel-forming multispanning membrane protein Sec61p/Sec61a and the tail-anchored Sss1p/Sec61?, which has been proposed to "clamp" the channel. We have analyzed the function of Sss1p using a series of domain mutants and found that both the cytosolic and transmembrane clamp domains of Sss1p are essential for protein translocation. Our data reveal that the cytosolic domain is required for Sec61p interaction but that the transmembrane clamp domain is required to complete activation of the translocon after precursor targeting to Sec61p. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

    Related items

    Showing items related by title, author, creator and subject.

    • Comprehensive overexpression analysis of cyclic-di-GMP signalling proteins in the phytopathogen Pectobacterium atrosepticum reveals diverse effects on motility and virulence phenotypes
      Tan, H.; West, J.; Ramsay, Joshua; Monson, R.; Griffin, J.; Toth, I.; Salmond, G. (2014)
      Bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous bacterial signalling molecule produced by diguanylate cyclases of the GGDEF-domain family. Elevated c-di-GMP levels or increased GGDEF protein ...
    • Molecular modeling of Bt Cry1Ac (DI-DII)-ASAL (Allium sativum lectin)-fusion protein and its interaction with aminopeptidase N (APN) receptor of Manduca sexta
      Tajne, S.; Sanam, R.; Gundla, R.; Gandhi, Neha; Mancera, Ricardo; Boddupally, D.; Vudem, D.; Khareedu, V. (2012)
      Genetic engineering of Bacillus thuringiensis (Bt) Cry proteins has resulted in the synthesis of various novel toxin proteins with enhanced insecticidal activity and specificity towards different insect pests. In this ...
    • The protein ontology project: Structured vocabularies for proteins
      Chang, Elizabeth; Sidhu, Amandeep; Sidhu, B.; Dillon, Tharam S. (2005)
      The rapid generation of accessible protein data sources has generated confusion over protein data representation. The protein ontology project seeks to provide a set of structured vocabularies for protein domains that can ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.