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    Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note

    Access Status
    Fulltext not available
    Authors
    Zeugolis, D.
    Li, B.
    Lareu, Ricky R.
    Chan, C.
    Raghunath, M.
    Date
    2008
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Zeugolis, D.I. and Li, B. and Lareu, R.R. and Chan, C.K. and Raghunath, M. 2008. Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note. Journal of Biomaterials Science - Polymer Edition. 19 (10): pp. 1307-1317.
    Source Title
    Journal of Biomaterials Science - Polymer Edition
    DOI
    10.1163/156856208786052344
    ISSN
    0920-5063
    URI
    http://hdl.handle.net/20.500.11937/25566
    Collection
    • Curtin Research Publications
    Abstract

    Collagen is the main component of the extra-cellular matrix and has been utilised for numerous clinical applications in many forms and products. However, since collagen remains a natural animal-derived biopolymer, variation between batches should be addressed and minimised to ensure reproducibility of the fabrication process. Recently, electro-spinning of collagen has been introduced as a leading technique for the production of bio-mimetic nano-scale scaffolds for tissue-engineering applications. However, no protocols are available that would allow comparisons of the quality of different collagen raw materials prior to the electro-spinning process. In order to bridge this gap we assessed the solubility of various freeze-dried collagens in 0.5 M acetic acid and analysed the solved collagen by gel electrophoresis. We show that raw material of limited solubility in acetic acid will not render high quality electro-spun nano-fibres using hexafluoropropanol. In particular, insoluble collagen directly failed to produce nano-fibres, collagen of reduced solubility produced fused nano-fibres with limited inter-nano-fibre space, whilst purified type-I collagen of high solubility produced smooth, reproducible nano-fibres. Gel electrophoresis confirmed the amount of solubility, as well as qualitative differences in terms of collagen cross-links and collagen types. We recommend this simple and fast step to save costs and to enhance control over the electro-spinning process of collagen. Furthermore, we believe that the solubility test should be introduced prior to any collagenous matrix preparation in order to ensure reproducibility and accuracy.

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