Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note
dc.contributor.author | Zeugolis, D. | |
dc.contributor.author | Li, B. | |
dc.contributor.author | Lareu, Ricky R. | |
dc.contributor.author | Chan, C. | |
dc.contributor.author | Raghunath, M. | |
dc.date.accessioned | 2017-01-30T12:49:07Z | |
dc.date.available | 2017-01-30T12:49:07Z | |
dc.date.created | 2014-02-25T20:00:39Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Zeugolis, D.I. and Li, B. and Lareu, R.R. and Chan, C.K. and Raghunath, M. 2008. Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note. Journal of Biomaterials Science - Polymer Edition. 19 (10): pp. 1307-1317. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/25566 | |
dc.identifier.doi | 10.1163/156856208786052344 | |
dc.description.abstract |
Collagen is the main component of the extra-cellular matrix and has been utilised for numerous clinical applications in many forms and products. However, since collagen remains a natural animal-derived biopolymer, variation between batches should be addressed and minimised to ensure reproducibility of the fabrication process. Recently, electro-spinning of collagen has been introduced as a leading technique for the production of bio-mimetic nano-scale scaffolds for tissue-engineering applications. However, no protocols are available that would allow comparisons of the quality of different collagen raw materials prior to the electro-spinning process. In order to bridge this gap we assessed the solubility of various freeze-dried collagens in 0.5 M acetic acid and analysed the solved collagen by gel electrophoresis. We show that raw material of limited solubility in acetic acid will not render high quality electro-spun nano-fibres using hexafluoropropanol. In particular, insoluble collagen directly failed to produce nano-fibres, collagen of reduced solubility produced fused nano-fibres with limited inter-nano-fibre space, whilst purified type-I collagen of high solubility produced smooth, reproducible nano-fibres. Gel electrophoresis confirmed the amount of solubility, as well as qualitative differences in terms of collagen cross-links and collagen types. We recommend this simple and fast step to save costs and to enhance control over the electro-spinning process of collagen. Furthermore, we believe that the solubility test should be introduced prior to any collagenous matrix preparation in order to ensure reproducibility and accuracy. | |
dc.publisher | Taylor & Francis | |
dc.subject | electro-spinning | |
dc.subject | Collagen nano-fibres | |
dc.subject | collagen solubility | |
dc.subject | SDS-PAGE | |
dc.title | Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note | |
dc.type | Journal Article | |
dcterms.source.volume | 19 | |
dcterms.source.number | 10 | |
dcterms.source.startPage | 1307 | |
dcterms.source.endPage | 1317 | |
dcterms.source.issn | 0920-5063 | |
dcterms.source.title | Journal of Biomaterials Science - Polymer Edition | |
curtin.department | ||
curtin.accessStatus | Fulltext not available |