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The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.
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Dol, Sharul sham; Salek, M.; Viegas, K.; Rinker, K.; Martinuzzi, R. (2010)Wall shear stress acting on arterial walls is an important hemodynamic force determining vessel health. A parallel-plate flow chamber with a 127 µm-thick flow channel is used as an in vitro system to study the fluid ...
Expansion and Hepatocytic Differentiation of Liver Progenitor Cells In Vivo Using a Vascularized Tissue Engineering Chamber in MiceForster, N.; Palmer, J.; Yeoh, G.; Ong, W.; Mitchell, G.; Slavin, J.; Tirnitz-Parker, Janina; Morrison, W. (2011)Current cell-based treatment alternatives to organ transplantation for liver failure remain unsatisfactory. Hepatocytes have a strong tendency to dedifferentiate and apoptose when isolated and maintained in culture. In ...
Investigation of the impact of nitrate injection to control sourcing problem in oil reservoir : benefit and side effects on steel materialsHalim, Amalia Yunita (2011)The successful control of reservoir souring by nitrate injection has been well documented in the literature. Recent interest has centred on how nitrate application can increase the corrosion risk in pipelines and metal ...