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    Boyden chamber

    Access Status
    Fulltext not available
    Authors
    Falasca, Marco
    Raimondi, C.
    Maffucci, T.
    Date
    2011
    Type
    Book Chapter
    
    Metadata
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    Citation
    Falasca, M. and Raimondi, C. and Maffucci, T. 2011. Boyden chamber, in Wells, C. and Parsons, M. (ed), Cell Migration: Developmental Methods and Protocols, pp. 87-95. London: Springer.
    Source Title
    Methods in Molecular Biology
    DOI
    10.1007/978-1-61779-207-6_7
    ISSN
    1064-3745
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/26064
    Collection
    • Curtin Research Publications
    Abstract

    The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.

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