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The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.
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Dol, Sharul sham; Salek, M.; Viegas, K.; Rinker, K.; Martinuzzi, R. (2010)Wall shear stress acting on arterial walls is an important hemodynamic force determining vessel health. A parallel-plate flow chamber with a 127 µm-thick flow channel is used as an in vitro system to study the fluid ...
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Corrective effects on airway epithelial function by human amniotic epithelial cells (HAEC) when treating cystic fibrosisKicic, Anthony; Ling, K.; Martinovich, K.; Garratt, L.; Looi, K.; Kicic-Starcevich, E.; Iosifidis, T.; Sutanto, E.; Tarran, R.; Cmielewski, P.; Donnelley, M.; Parsons, D.; Stick, S.; Moodley, Y. (2015)Background: Cystic fibrosis (CF) remains the most common life-shortening genetic disease affecting children. The defective protein product of the affected gene, cystic fibrosis transmembrane conductance regulator (CFTR), ...