The effect of physicochemical factors on the self-association of HMGB1: A surface plasmon resonance study
MetadataShow full item record
© 2016 Elsevier B.V. HMGB1 triggers proinflammatory reactions by interacting extracellularly with various receptors. HMGB1 also acts in the nucleus by interacting with DNA and controlling DNA transcription, a process which involves its self-association. The self-association of HMGB1 was characterized using surface plasmon resonance (SPR). A dimer/tetramer binding model was developed that provided a good fit to the SPR sensorgrams and enabled the kinetics of self-association of different HMGB1 oligomers to be evaluated under a variety of physicochemical conditions. The formation of HMGB1 tetramers, and not dimers, was strongly influenced by ionic strength. HMGB1 self-association increased as the pH was decreased from 7.4 to 4.8 but was abolished at pH 4.0, suggesting the involvement of acidic amino acids of HMGB1 in its self-association. HMGB1 dimers were found to predominate in the absence of zinc, but addition of zinc promoted the formation of HMGB1 tetramers. More reducing conditions favored dimerization but diminished tetramer formation. In contrast, oxidizing conditions favored tetramer formation. Physicochemical factors modulate the extent of self-association of HMGB1. We speculate that HMGB1 dimers may preferentially bind DNA, whereas HMGB1 tetramers may promote inflammatory responses by binding to RAGE and TLRs. The self-association of HMGB1, regulated by variations of physicochemical factors, may influence its roles in DNA rearrangement and regulation of pathophysiological diseases.
Showing items related by title, author, creator and subject.
Formation of styrene monomer, dimer and trimer in the primary volatiles produced from polystyrene pyrolysis in a wire-mesh reactorZhou, J.; Qiao, Y.; Wang, W.; Leng, E.; Huang, J.; Yu, Yun; Xu, M. (2016)© 2016 Elsevier Ltd.This paper reports the formation of styrene monomer, dimer and trimer in the primary volatiles during fast pyrolysis of polystyrene in a wire-mesh reactor where secondary reactions of primary pyrolysis ...
Pharmaceutical Aspects of the Recombinant Human Serum Albumin Dimer: Structural Characteristics, Biological Properties, and Medical ApplicationsTaguchi, K.; Chuang, Victor; Maruyama, T.; Otagiri, M. (2012)Human serum albumin is the most abundant protein in the blood. It is clinically used in the treatment of severe hypoalbuminemia and as a plasma expander. The use of albumins as a carrier for drugs is currently being ...
Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycleGorzin, A.; Ramsland, Paul; Tachedjian, G.; Gowans, E. (2012)The NS2 protein of hepatitis C virus (HCV) plays a critical role in virus morphogenesis and infectivity. The crystal structure of the C-terminus of the NS2 protein (NS2 Pro) from the H77 strain ...