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    Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines

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    Fulltext not available
    Authors
    Kanafi, M.
    Mamidi, M.
    Sureshbabu, S.
    Shahani, P.
    Bhawna, C.
    Warrier, Sudha
    Bhonde, R.
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Kanafi, M. and Mamidi, M. and Sureshbabu, S. and Shahani, P. and Bhawna, C. and Warrier, S. and Bhonde, R. 2014. Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines. Biotechnology Letters. 37 (1): pp. 227-233.
    Source Title
    Biotechnology Letters
    DOI
    10.1007/s10529-014-1662-7
    ISSN
    0141-5492
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/27481
    Collection
    • Curtin Research Publications
    Abstract

    To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut-2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research.

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