Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines
dc.contributor.author | Kanafi, M. | |
dc.contributor.author | Mamidi, M. | |
dc.contributor.author | Sureshbabu, S. | |
dc.contributor.author | Shahani, P. | |
dc.contributor.author | Bhawna, C. | |
dc.contributor.author | Warrier, Sudha | |
dc.contributor.author | Bhonde, R. | |
dc.date.accessioned | 2017-01-30T12:59:18Z | |
dc.date.available | 2017-01-30T12:59:18Z | |
dc.date.created | 2015-10-29T04:10:01Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Kanafi, M. and Mamidi, M. and Sureshbabu, S. and Shahani, P. and Bhawna, C. and Warrier, S. and Bhonde, R. 2014. Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines. Biotechnology Letters. 37 (1): pp. 227-233. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/27481 | |
dc.identifier.doi | 10.1007/s10529-014-1662-7 | |
dc.description.abstract |
To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut-2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research. | |
dc.publisher | Kluwer Academic Publishers | |
dc.title | Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines | |
dc.type | Journal Article | |
dcterms.source.issn | 0141-5492 | |
dcterms.source.title | Biotechnology Letters | |
curtin.department | School of Biomedical Sciences | |
curtin.accessStatus | Fulltext not available |
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