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    DNA from soil mirrors plant taxonomic and growth form diversity

    Access Status
    Fulltext not available
    Authors
    Yoccoz, N.
    Brathen, K.
    Gielly, L.
    Haile, James
    Edwards, M.
    Goslar, T.
    Von Stedingk, H.
    Brysting, A.
    Coissac, E.
    Pompanon, F.
    Sonstebo, J.
    Miquel, C.
    Valentini, A.
    De Bello, F.
    Chave, J.
    Thuiller, W.
    Wincker, P.
    Cruaud, C.
    Gavory, F.
    Rasmussen, M.
    Gilbert, Thomas
    Orlando, L.
    Brochmann, C.
    Willerslev, E.
    Taberlet, P.
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    Yoccoz, N. and Brathen, K. and Gielly, L. and Haile, J. and Edwards, M. and Goslar, T. and Von Stedingk, H. et al. 2012. DNA from soil mirrors plant taxonomic and growth form diversity. Molecular Ecology. 21: pp. 3647-3655.
    Source Title
    Molecular Ecology
    ISSN
    09621083
    URI
    http://hdl.handle.net/20.500.11937/27755
    Collection
    • Curtin Research Publications
    Abstract

    Ecosystems across the globe are threatened by climate change and human activities. New rapid survey approaches for monitoring biodiversity would greatly advance assessment and understanding of these threats. Taking advantage of next-generation DNA sequencing, we tested an approach we call metabarcoding: high-throughput and simultaneous taxa identification based on a very short (usually <100 base pairs) but informative DNA fragment. Short DNA fragments allow the use of degraded DNA from environmental samples. All analyses included amplification using plant-specific versatile primers, sequencing and estimation of taxonomic diversity. We tested in three steps whether degraded DNA from dead material in soil has the potential of efficiently assessing biodiversity in different biomes. First, soil DNA from eight boreal plant communities located in two different vegetation types (meadow and heath) was amplified. Plant diversity detected from boreal soil was highly consistent with planttaxonomic and growth form diversity estimated from conventional above-ground surveys. Second, we assessed DNA persistence using samples from formerly cultivated soils in temperate environments. We found that the number of crop DNA sequences retrieved strongly varied with years since last cultivation, and crop sequences were absent from nearby, uncultivated plots. Third, we assessed the universal applicability of DNA metabarcoding using soil samples from tropical environments: a large proportion of species and families from the study site were efficiently recovered. The results openunprecedented opportunities for large-scale DNA-based biodiversity studies across a range of taxonomic groups using standardized metabarcoding approaches.

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