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    Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding

    87266.pdf (1.053Mb)
    Access Status
    Open access
    Authors
    Van Der Heyde, Mieke
    Bunce, Michael
    Wardell-Johnson, Grant
    Fernandes, Kristen
    White, Nicole
    Nevill, Paul
    Date
    2020
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    van der Heyde, M. and Bunce, M. and Wardell-Johnson, G. and Fernandes, K. and White, N.E. and Nevill, P. 2020. Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding. Molecular Ecology Resources. 20 (3): pp. 732-745.
    Source Title
    Molecular Ecology Resources
    DOI
    10.1111/1755-0998.13148
    ISSN
    1755-098X
    Faculty
    Faculty of Science and Engineering
    School
    School of Molecular and Life Sciences (MLS)
    Funding and Sponsorship
    http://purl.org/au-research/grants/arc/IC150100041
    Remarks

    This is the peer reviewed version of the following article: van der Heyde, M, Bunce, M, Wardell-Johnson, G, Fernandes, K, White, NE, Nevill, P. Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding. Mol Ecol Resour. 2020; 20: 732– 745, which has been published in final form at https://doi.org/10.1111/1755-0998.13148. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

    URI
    http://hdl.handle.net/20.500.11937/87446
    Collection
    • Curtin Research Publications
    Abstract

    Biological surveys based on visual identification of the biota are challenging, expensive and time consuming, yet crucial for effective biomonitoring. DNA metabarcoding is a rapidly developing technology that can also facilitate biological surveys. This method involves the use of next generation sequencing technology to determine the community composition of a sample. However, it is uncertain as to what biological substrate should be the primary focus of metabarcoding surveys. This study aims to test multiple sample substrates (soil, scat, plant material and bulk arthropods) to determine what organisms can be detected from each and where they overlap. Samples (n = 200) were collected in the Pilbara (hot desert climate) and Swan Coastal Plain (hot Mediterranean climate) regions of Western Australia. Soil samples yielded little plant or animal DNA, especially in the Pilbara, probably due to conditions not conducive to long-term preservation. In contrast, scat samples contained the highest overall diversity with 131 plant, vertebrate and invertebrate families detected. Invertebrate and plant sequences were detected in the plant (86 families), pitfall (127 families) and vane trap (126 families) samples. In total, 278 families were recovered from the survey, 217 in the Swan Coastal Plain and 156 in the Pilbara. Aside from soil, 22%–43% of the families detected were unique to the particular substrate, and community composition varied significantly between substrates. These results demonstrate the importance of selecting appropriate metabarcoding substrates when undertaking terrestrial surveys. If the aim is to broadly capture all biota then multiple substrates will be required.

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