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dc.contributor.authorTan, M.
dc.contributor.authorAgyei, D.
dc.contributor.authorPan, S.
dc.contributor.authorDanquah, Michael
dc.date.accessioned2017-01-30T13:08:14Z
dc.date.available2017-01-30T13:08:14Z
dc.date.created2016-06-05T19:31:14Z
dc.date.issued2015
dc.identifier.citationTan, M. and Agyei, D. and Pan, S. and Danquah, M. 2015. Parametric investigation of batch adsorption of proteins onto Polymeric Particles. Current Pharmaceutical Biotechnology. 16 (9): pp. 816-822.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/28912
dc.description.abstract

Background: Effective bimolecular adsorption of proteins onto solid matrices is characterized by in-depth understanding of the biophysical features essential to optimize the adsorption performance, Results: The adsorption of bovine serum albumin (BSA) onto anion-exchange Q-sepharose solid particulate support was investigated in batch adsorption experiments, Adsorption kinetics and isotherms were developed as a function of key industrially relevant parameters such as polymer loading, stirring speed, buffer pH, protein concentration and the state of protein dispersion (solid/aqueous) in order to optimize binding performance and adsorption capacity, Experimental results showed that the first order rate constant is higher at higher stirring speed, higher polymer loading, and under alkaline conditions, with a corresponding increase in equilibrium adsorption capacity, Increasing the stirring speed and using aqueous dispersion protein system increased the adsorption rate, but the maximum protein adsorption was unaffected, Regardless of the stirring speed, the adsorption capacity of the polymer was 2.8 mg/ml, However, doubling the polymer loading increased the adsorption capacity to 9.4 mg/ml, Conclusions: The result demonstrates that there exists a minimum amount of polymer loading required to achieve maximum protein adsorption capacity under specific process conditions.

dc.relation.urihttp://www.ingentaconnect.com/content/ben/cpb/2015/00000016/00000009/art00008?crawler=true
dc.titleParametric investigation of batch adsorption of proteins onto Polymeric Particles
dc.typeJournal Article
dcterms.source.volume16
dcterms.source.number9
dcterms.source.startPage816
dcterms.source.endPage822
dcterms.source.issn1389-2010
dcterms.source.titleCurrent Pharmaceutical Biotechnology
curtin.departmentCurtin Sarawak
curtin.accessStatusFulltext not available


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