Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

    Access Status
    Open access via publisher
    Authors
    Monecke, S.
    Muller, E.
    Buechler, J.
    Rejman, J.
    Stieber, B.
    Akpaka, P.
    Bandt, D.
    Burris, R.
    Coombs, Geoffrey
    Hidalgo-Arroyo, A.
    Hughes, P.
    Kearns, A.
    Abos, S.
    Pichon, B.
    Skanki, L.
    Soderquist, B.
    Ehricht, R.
    Date
    2013
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Monecke, S. and Muller, E. and Buechler, J. and Rejman, J. and Stieber, B. and Akpaka, P. and Bandt, D. et al. 2013. Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies. Journal of Clinical Microbiology. 51 (2): pp. 487-495.
    Source Title
    Journal of Clinical Microbiology
    DOI
    10.1128/JCM.02285-12
    ISSN
    00951137
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/29059
    Collection
    • Curtin Research Publications
    Abstract

    Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spotted on protein microarrays and screened using different lukF-PV preparations and detection antibodies. This led to the identification of the optimal antibody combination that was then used to establish a lateral flow assay. This test was used to detect PVL in S. aureus cultures. The detection limit of the assay with purified native and recombinant antigens was determined to be around 1 ng/ml. Overnight cultures from various solid and liquid media proved suitable for PVL detection. Six hundred strains and clinical isolates from patients from America, Europe, Australia, Africa, and the Middle East were tested. Isolates were genotyped in parallel by DNA microarray hybridization for confirmation of PVL status and assignment to clonal complexes. The sensitivity, specificity, and positive and negative predictive values of the assay in this trial were 99.7, 98.3, 98.4, and 99.7%, respectively. A total of 302 clinical isolates and reference strains were PVL positive and were assigned to 21 different clonal complexes. In summary, the lateral flow test allows rapid and economical detection of PVL in a routine bacteriology laboratory. As the test utilizes cultures from standard media and does not require sophisticated equipment, it can be easily integrated into a laboratory's workflow and might contribute to timely therapy of PVL-associated infections.

    Related items

    Showing items related by title, author, creator and subject.

    • Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.
      Gao, K.; Chen, Younan; Wei, L.; Li, S.; Jin, X.; Cong, C.; Yuan, Y.; Long, D.; Li, Y.; Cheng, J.; Lu, Y. (2008)
      We investigated the mechanism underlying the inhibitory effect of rat mesenchymal stem cells (MSCs) on non-specific mitogen-stimulated lymphocytes (LCs) and lymphoblasts (LBs). We used MSCs of passages 2-8 prepared from ...
    • Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, june 2013
      Perera, R.; Wang, P.; Gomaa, M.; El-Shesheny, R.; Kandeil, A.; Bagato, O.; Siu, L.; Shehata, M.; Kayed, A.; Moatasim, Y.; Li, M.; Poon, L.; Guan, Y.; Webby, R.; Ali, Mohammed; Peiris, J.; Kayali, G. (2013)
      We describe a novel spike pseudoparticle neutralisation assay (ppNT) for seroepidemiological studies on Middle East respiratory syndrome coronavirus (MERSCoV) and apply this assay together with conventional microneutralisation ...
    • Expression and modulation of tissue factor and tissue factor pathway inhibitor in an endothelial cell based model
      Ellery, Paul E. R. (2008)
      Haemostasis is a complex physiological process involving cellular and plasma protein components that interact to keep the blood fluid under normal conditions and prevent blood loss after vessel injury by promoting clot ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.