Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.

    Access Status
    Fulltext not available
    Authors
    Gao, K.
    Chen, Younan
    Wei, L.
    Li, S.
    Jin, X.
    Cong, C.
    Yuan, Y.
    Long, D.
    Li, Y.
    Cheng, J.
    Lu, Y.
    Date
    2008
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Gao, K. and Chen, Y. and Wei, L. and Li, S. and Jin, X. and Cong, C. and Yuan, Y. et al. 2008. Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.. Cell Biochemistry and Function. 26 (8): pp. 900-907.
    Source Title
    Cell Biochemistry and Function
    DOI
    10.1002/cbf.1523
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/30702
    Collection
    • Curtin Research Publications
    Abstract

    We investigated the mechanism underlying the inhibitory effect of rat mesenchymal stem cells (MSCs) on non-specific mitogen-stimulated lymphocytes (LCs) and lymphoblasts (LBs). We used MSCs of passages 2-8 prepared from Sprague-Dawley (SD) rats. LCs were isolated from the spleens of SD rats. Mixed LCs reactions of mitomycin C-treated MSCs with concanavalin A (ConA)-stimulated LCs or LBs were performed, and the proliferation inhibition effect was tested by MTS assay. The cytotoxicity of MSCs against naïve and ConA-stimulated LBs was detected, after co-culturing for 24 h, by lactate dehydrogenase release assay. The rate of apoptosis of ConA-stimulated LBs was measured by flow cytometry after incubation with MSCs for 9 h in the ratio 10:1. The MSCs were treated with Fas ligand (FasL), transforming growth factor (TGF)-beta, and interleukin (IL)-10 blocking antibodies and co-cultured with ConA-stimulated LBs to observe the apoptosis and growth inhibitory effect. The main outcomes were bone marrow-derived adherent CD29+, CD44+, CD45-, CD54+, CD95+, and SH-2+ MSCs. FasL, TGF-beta, and IL-10 production by MSCs were visualized by immunocytochemical analysis. MSCs exhibited a dose-dependent growth inhibitory effect on ConA-stimulated LCs and LBs. When treated with anti-FasL and anti-IL-10 blocking antibodies, the inhibitory effect of MSCs on LBs proliferation, and the effect of apoptosis induction on LBs decreased. Anti-TGF-beta blocking antibody treatment did not significantly influence MSCs. Therefore, the inhibitory effects of MSCs against activated LBs were significantly stronger than that against naïve LCs. FasL and IL-10, rather than TGF-beta, play important roles in the immunosuppressive effects of MSCs.

    Related items

    Showing items related by title, author, creator and subject.

    • Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury
      Lu, Y.; Jin, X.; Chen, Younan; Li, S.; Yuan, Y.; Mai, G.; Tian, B.; Long, D.; Zhang, J.; Zeng, L.; Li, Y.; Cheng, J. (2010)
      Hypoxia/reoxygenation (H/R)-induced injury is the key factor associated with islet graft dysfunction. This study aims to examine the effect of mesenchymal stem cells (MSCs) on islet survival and insulin secretion under ...
    • The impact of transcranial direct current stimulation on inhibitory control in young adults
      Loftus, Andrea; Yalcin, O.; Baughman, Frank; Vanman, E.; Hagger, Martin (2015)
      Background: There is increasing evidence that the dorso-lateral prefrontal cortex (DLPFC), a brain region related to reward and motivational processes, is involved in effective response inhibition and that decreased ...
    • Mechanims of lactose crystallisation
      Dincer, Tuna (2000)
      Lactose is the major carbohydrate in milk. The presence of lactose in whey constitutes a significant pollution problem for dairy factories. At the same time, there is an increasing market for high quality crystalline ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.