Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    The podosphaera fusca TUB2 gene, a molecular "swiss army knife" with multiple applications in powdery mildew research

    Access Status
    Fulltext not available
    Authors
    Vela-Corcía, David
    Bellón-Góme, Davinia
    Lopez-Ruiz, Francisco
    Tores, Juan
    Perez-Garcia, Alejandro
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Vela-Corcía, David and Bellón-Góme, Davinia and Lopez-Ruiz, Francisco and Tores, Juan and Perez-Garcia, Alejandro. 2014. The podosphaera fusca TUB2 gene, a molecular "Swiss Army knife" with multiple applications in powdery mildew research. Fungal Biology. 118 (2): pp. 228-241.
    Source Title
    Fungal Biology
    DOI
    10.1016/j.funbio.2013.12.001
    ISSN
    1878-6146
    URI
    http://hdl.handle.net/20.500.11937/29198
    Collection
    • Curtin Research Publications
    Abstract

    The powdery mildew fungus Podosphaera fusca (synonym Podosphaera xanthii) is the main causal agent of cucurbit powdery mildew and one of the most important limiting factors for cucurbit production worldwide. Despite the fungus' economic importance, very little is known about the physiological and molecular processes involved in P. fusca biology and pathogenesis. In this study, we isolated and characterised the β-tubulin-encoding gene of P. fusca (PfTUB2) to develop molecular tools with different applications in powdery mildew research. PfTUB2 is predicted to encode a protein of 447 amino acid residues. The coding region is interrupted by six introns that occur at approximately the same positions as the introns present in other fungal TUB2-like genes. Once cloned, the PfTUB2 sequence information was used in different applications. Our results showed that the TUB2 gene is a good marker for molecular phylogenetics in powdery mildew fungi but it is unsuitable for the analysis of intraspecific diversity in P. fusca. The expression of PfTUB2 was proven to be stable in different temperature conditions, supporting its use as a reference gene in quantitative gene expression studies. Furthermore, an allele-specific PCR assay for the detection of resistance to methyl-2-benzimidazole carbamate (MBC) fungicides in P. fusca was developed based on the correlation between the single amino acid change E198A in β-tubulin and the MBC resistance phenotype. Lastly, PfTUB2 was used as a target gene in the development of a high-throughput method to quantify fungal growth in plant tissues.

    Related items

    Showing items related by title, author, creator and subject.

    • Genome Expansion and Gene Loss in Powdery Mildew Fungi Reveal Tradeoffs in Extreme Parasitism
      Spanu, P.; Abbott, J.; Amselem, J.; Burgis, T.; Soanes, D.; Stüber, K.; Ver Loren van Themaat, E.; Brown, J.; Butcher, S.; Gurr, S.; Lebrun, M.; Ridout, C.; Schulze-Lefert, P.; Talbot, N.; Ahmadinejad, N.; Ametz, C.; Barton, G.; Benjdia, M.; Bidzinski, P.; Bindschedler, L.; Both, M.; Brewer, M.; Cadle-Davidson, L.; Cadle-Davidson, M.; Collemare, J.; Cramer, R.; Frenkel, O.; Godfrey, D.; Harriman, J.; Hoede, C.; King, B.; Klages, S.; Kleemann, J.; Knoll, D.; Koti, P.; Kreplak, J.; Lopez-Ruiz, Fran; Lu, X.; Maekawa, T.; Mahanil, S.; Micali, C.; Milgroom, M.; Montana, G.; Noir, S.; O’Connell, R.; Oberhaensli, S.; Parlange, F.; Pedersen, C.; Quesneville, H.; Reinhardt, R.; Rott, M.; Sacristán, S.; Schmidt, S.; Schön, M.; Skamnioti, P.; Sommer, H.; Stephens, A.; Takahara, H.; Thordal-Christensen, H.; Vigouroux, M.; Weßling, R.; Wicker, T.; Panstruga, R. (2010)
      Powdery mildews are phytopathogens whose growth and reproduction are entirely dependent on living plant cells. The molecular basis of this life-style, obligate biotrophy, remains unknown. We present the genome analysis ...
    • First occurence of cucurbit powdery mildew caused by race 3-5 of Podosphaera fusca (synonym Podosphaera xanthii) in Spain
      Torés, J.; Sánchez-Pulido, J.; Lopez-Ruiz, Fran; de Vicente, A.; Pérez-García, A. (2009)
      A new race of cucurbit powdery mildew was observed for the first time on melon (Cucumis melo) in three research greenhouses in the Axarquia area of southern Spain during the spring of 2008. Fungal growth appeared as white ...
    • Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection
      Bus, V.; Bassett, H.; Bowatte, D.; Chagné, D.; Ranatunga, C.; Ulluwishewa, Dulantha; Wiedow, C.; Gardiner, S. (2010)
      Apple is host to a wide range of pests and diseases, with several of these, such as apple scab, powdery mildew and woolly apple aphid, being major causes of damage in most areas around the world. Resistance breeding is ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.