Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface
Embargo Lift Date
MetadataShow full item record
© 2016 Elsevier Ltd. Electrochemical characterization of hen egg white lysozyme (HEWL) at a glycerol-modified interface between two immiscible electrolyte solutions (ITIES) was conducted using a microporous silicon membrane-supported gelled-1,6-dichlorohexane|water-glycerol interface. The electrochemical response of HEWL under these conditions is of interest for the system's potential application to the formation of isorefractive emulsified-ITIES, which offer practical opportunities for spectrophotometric analysis of interfacial processes. Importantly, the voltammetric signature for HEWL seen under glycerol-rich conditions was similar but with some differences from that for glycerol-free conditions. Specifically, the potential at which facilitated transfer of the organic phase electrolyte anion tetrakis-(4-chlorophenyl) borate (TPBCl-) occurred was shifted to lower potential with increasing glycerol concentration. However, features in the voltammetry associated with adsorption/desorption processes were observed to remain constant. The simple ion transfer response of tetraethylammonium cations (TEA+) at the same glycerol-modified ITIES provides insight into the nature of changes that determine the atypical HEWL signature. Lower ion transfer current with respect to increasing glycerol concentration and a shift in transfer potential were the key findings here. The results indicate that the electrochemistry which determines the HEWL signature is similar in environments that are rich in glycerol or purely aqueous.
Showing items related by title, author, creator and subject.
Electrochemical behaviour of hen-egg-white lysozyme at the polarised water/1,2-dichloroethane interfaceScanlon, M.; Jennings, E.; Arrigan, Damien (2009)The electrochemical behaviour of hen-egg-white lysozyme (HEWL) was studied at the polarizedwater/1,2-dichloroethane interface. The voltammetric ion-transfer response was found to bedependent on the pH and ionic strength ...
Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozymeFelisilda, B.; Alvarez De Eulate, Eva; Arrigan, Damien (2015)Ion transfer at aqueous-organogel interfaces enables the non-redox detection of ions and ionisable species by voltammetry. In this study, a non-thermal method for preparation of an organogel was employed and used for the ...
Adsorptive stripping voltammetry of hen-egg-white-lysozyme via adsorption-desorption at an array of liquid-liquid microinterfacesAlvarez de Eulate, E.; Arrigan, Damien (2012)Electrochemical adsorption and voltammetry of hen-egg-white-lysozyme (HEWL) was studied at an array of microinterfaces between two immiscible electrolyte solutions (µITIES). Adsorption of the protein was achieved at an ...