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dc.contributor.authorAusten, Benjamin
dc.contributor.authorArrigan, Damien
dc.date.accessioned2017-01-30T10:27:13Z
dc.date.available2017-01-30T10:27:13Z
dc.date.created2016-11-15T19:30:19Z
dc.date.issued2016
dc.date.submitted2016-11-16
dc.identifier.citationAusten, B. and Arrigan, D. 2016. Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface. Electrochimica Acta. 221: pp. 62-69.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/2940
dc.identifier.doi10.1016/j.electacta.2016.10.098
dc.description.abstract

Electrochemical characterization of hen egg white lysozyme (HEWL) at a glycerol-modified interface between two immiscible electrolyte solutions (ITIES) was conducted using a microporous silicon membrane-supported gelled-1,6-dichlorohexane|water-glycerol interface. The electrochemical response of HEWL under these conditions is of interest for the system's potential application to the formation of isorefractive emulsified-ITIES, which offer practical opportunities for spectrophotometric analysis of interfacial processes. Importantly, the voltammetric signature for HEWL seen under glycerol-rich conditions was similar but with some differences from that for glycerol-free conditions. Specifically, the potential at which facilitated transfer of the organic phase electrolyte anion tetrakis-(4-chlorophenyl) borate (TPBCl-) occurred was shifted to lower potential with increasing glycerol concentration. However, features in the voltammetry associated with adsorption/desorption processes were observed to remain constant. The simple ion transfer response of tetraethylammonium cations (TEA+) at the same glycerol-modified ITIES provides insight into the nature of changes that determine the atypical HEWL signature. Lower ion transfer current with respect to increasing glycerol concentration and a shift in transfer potential were the key findings here. The results indicate that the electrochemistry which determines the HEWL signature is similar in environments that are rich in glycerol or purely aqueous.

dc.publisherPergamon
dc.titleElectrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interface
dc.typeJournal Article
dcterms.dateSubmitted2016-11-16
dcterms.source.volume221
dcterms.source.startPage62
dcterms.source.endPage69
dcterms.source.issn0013-4686
dcterms.source.titleElectrochimica Acta
curtin.digitool.pid246588
curtin.pubStatusIn Press
curtin.refereedTRUE
curtin.departmentNanochemistry Research Institute
curtin.identifier.scriptidPUB-SE-DAC-DA-15192
curtin.identifier.elementsidELEMENTS-140039
curtin.accessStatusOpen access


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