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    Identification of sex specific DNA regions in the snake genome using a subtractive hybridisation technique

    134184_16744_harris572.pdf (277.6Kb)
    Access Status
    Open access
    Authors
    Harris, R.
    Groth, David
    Ledger, J.
    Lee, C.
    Date
    2009
    Type
    Conference Paper
    
    Metadata
    Show full item record
    Citation
    Harris, R., and Groth, D., and Ledger, J., and Lee, C. 2009. Identification of sex specific DNA regions in the snake genome using a subtractive hybridization technique, in the Proceedings of the 18th Conference of the Association for the Advancement of Animal Breeding and Genetics, pp: 572 - 575. Barossa Valley South Australia: AAABG.
    Source Title
    Proceedings of the Eighteenth AAABG conference: Matching Genetics and Environment: a New look at an old topic
    Source Conference
    Association for the advancement of animal breeding and genetics
    Additional URLs
    http://www.aaabg.org/proceedings18/index.html
    ISSN
    13283227
    Faculty
    Faculty of Health Sciences
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/31681
    Collection
    • Curtin Research Publications
    Abstract

    Karyotypic studies have shown that a ZZ/ZW sex chromosome system is used by snakes, which chromosomally resembles the ZZ/ZW system used by birds. However genetic studies have shown that SOX 3; the sex determining gene in mammals and DMRT1; which is the hypothesised sex determining gene in birds, are both located on autosomal chromosomes in the snake. Therefore it has been suggested that the snake sex chromosome system is unique and has evolved independently of the bird?s system. This paper describes a subtractive hybridization method, using physical separation of biotinylated 'driver' DNA. The novel application of this technique was its use in identifying sex specific DNA regions within the genome of the Australian python; Morelia spilota imbricate. Female DNA enrichment was achieved using this technique and resulted in the identification of two non-sex specific repeating elements. The conclusion from this work is the identification of female specific DNA in snakes requires further subtractive hybridization enrichment and a more efficient screening procedure.

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