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dc.contributor.authorHarris, R.
dc.contributor.authorGroth, David
dc.contributor.authorLedger, J.
dc.contributor.authorLee, C.
dc.contributor.editorAAABG Secretary
dc.date.accessioned2017-01-30T13:26:50Z
dc.date.available2017-01-30T13:26:50Z
dc.date.created2010-03-16T20:02:08Z
dc.date.issued2009
dc.identifier.citationHarris, R., and Groth, D., and Ledger, J., and Lee, C. 2009. Identification of sex specific DNA regions in the snake genome using a subtractive hybridization technique, in the Proceedings of the 18th Conference of the Association for the Advancement of Animal Breeding and Genetics, pp: 572 - 575. Barossa Valley South Australia: AAABG.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/31681
dc.description.abstract

Karyotypic studies have shown that a ZZ/ZW sex chromosome system is used by snakes, which chromosomally resembles the ZZ/ZW system used by birds. However genetic studies have shown that SOX 3; the sex determining gene in mammals and DMRT1; which is the hypothesised sex determining gene in birds, are both located on autosomal chromosomes in the snake. Therefore it has been suggested that the snake sex chromosome system is unique and has evolved independently of the bird?s system. This paper describes a subtractive hybridization method, using physical separation of biotinylated 'driver' DNA. The novel application of this technique was its use in identifying sex specific DNA regions within the genome of the Australian python; Morelia spilota imbricate. Female DNA enrichment was achieved using this technique and resulted in the identification of two non-sex specific repeating elements. The conclusion from this work is the identification of female specific DNA in snakes requires further subtractive hybridization enrichment and a more efficient screening procedure.

dc.publisherAssociation for the advancement of animal breeding and genetics
dc.relation.urihttp://www.aaabg.org/proceedings18/index.html
dc.titleIdentification of sex specific DNA regions in the snake genome using a subtractive hybridisation technique
dc.typeConference Paper
dcterms.source.startPage572
dcterms.source.endPage575
dcterms.source.issn13283227
dcterms.source.titleProceedings of the Eighteenth AAABG conference: Matching Genetics and Environment: a New look at an old topic
dcterms.source.seriesProceedings of the Eighteenth AAABG conference: Matching Genetics and Environment: a New look at an old topic
dcterms.source.conferenceAssociation for the advancement of animal breeding and genetics
dcterms.source.conference-start-dateSep 29 2009
dcterms.source.conferencelocationBarossa Valley South Australia
dcterms.source.placeUniversity of Adelaide SA 5371
curtin.accessStatusOpen access
curtin.facultyFaculty of Health Sciences
curtin.facultySchool of Biomedical Sciences


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