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dc.contributor.authorSun, W.
dc.contributor.authorKurniawan, N.
dc.contributor.authorKumar, Alan Prem
dc.contributor.authorRajagopalan, R.
dc.contributor.authorLim, C.
dc.date.accessioned2017-01-30T13:31:24Z
dc.date.available2017-01-30T13:31:24Z
dc.date.created2015-04-09T09:08:00Z
dc.date.issued2014
dc.identifier.citationSun, W. and Kurniawan, N. and Kumar, A.P. and Rajagopalan, R. and Lim, C. 2014. Effects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration. Cellular and Molecular Bioengineering. 7 (2): pp. 205-217.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/32537
dc.identifier.doi10.1007/s12195-014-0324-0
dc.description.abstract

The migration of cells is fundamental to a number of physiological/pathological processes, ranging from embryonic development, tissue regeneration to cancer metastasis. Current research on cell migration is largely based on simplified in vitro models that assume a homogeneous microenvironment and overlook the modification of extracellular matrix (ECM) by the cells. To address this shortcoming, we developed a nested three-dimensional (3D) collagen hydrogel model mimicking the connective tissue confronted by highly malignant breast cancer cells, MDA-MB-231. Strikingly, our findings revealed two distinct cell migration patterns: a rapid and directionally persistent collective migration of the leader cells and a more randomized migration in the regions that have previously been significantly modified by cells. The cell-induced modifications, which typically include clustering and alignment of fibers, effectively segmented the matrix into smaller sub-regions. Our results suggest that in an elastic 3D matrix, the presence of adjacent cells that have modified the matrix may in fact become physical hurdle to a migrating cell. Furthermore, our study emphasizes the need for a micromechanical understanding in the context of cancer invasion that allows for cell-induced modification of ECM and a heterogeneous cell migration.

dc.publisherSpringer New York LLC
dc.subjectCell contraction
dc.subject3D extracellular matrix
dc.subjectCollective cell migration
dc.subjectCancer invasion
dc.subjectMatrix remodeling
dc.titleEffects of Migrating Cell-Induced Matrix Reorganization on 3D Cancer Cell Migration
dc.typeJournal Article
dcterms.source.volume7
dcterms.source.number2
dcterms.source.startPage205
dcterms.source.endPage217
dcterms.source.issn1865-5025
dcterms.source.titleCellular and Molecular Bioengineering
curtin.accessStatusFulltext not available


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