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    The Influence of Stabilized Deconjugated Ursodeoxycholic Acid on Polymer-Hydrogel System of Transplantable NIT-1 Cells

    237830_237830.pdf (1.004Mb)
    Access Status
    Open access
    Authors
    Mooranian, A.
    Negrulj, R.
    Al-Salami, Hani
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Mooranian, A. and Negrulj, R. and Al-Salami, H. 2016. The Influence of Stabilized Deconjugated Ursodeoxycholic Acid on Polymer-Hydrogel System of Transplantable NIT-1 Cells. Pharmaceutical Research. 33 (5): pp. 1182-1190.
    Source Title
    Pharmaceutical Research
    DOI
    10.1007/s11095-016-1863-y
    ISSN
    0724-8741
    School
    School of Pharmacy
    Remarks

    The final publication is available at Springer http://doi.org/10.1007/s11095-016-1863-y

    URI
    http://hdl.handle.net/20.500.11937/33902
    Collection
    • Curtin Research Publications
    Abstract

    Purpose: The encapsulation of pancreatic ß-cells in biocompatible matrix has generated great interest in diabetes treatment. Our work has shown improved microcapsules when incorporating the bile acid ursodeoxycholic acid (UDCA), in terms of morphology and cell viability although cell survival remained low. Thus, the study aimed at incorporating the polyelectrolytes polyallylamine (PAA) and poly-l-ornithine (PLO), with the polymer sodium alginate (SA) and the hydrogel ultrasonic gel (USG) with UDCA and examined cell viability and functionality post microencapsulation. Methods: Microcapsules without (control) and with UDCA (test) were produced using 1% PLO, 2.5% PAA, 1.8% SA and 4.5% USG. Pancreatic ß-cells were microencapsulated and the microcapsules’ morphology, surface components, cellular and bile acid distribution, osmotic and mechanical stability as well as biocompatibilities, insulin production, bioenergetics and the inflammatory response were tested. Results: Incorporation of UDCA at 4% into a PLO-PAA-SA formulation system increased cell survival (p < 0.01), insulin production (p < 0.01), reduced the inflammatory profile (TNF-a, IFN-?, IL-6 and IL-1ß; p < 0.01) and improved the microcapsule physical and mechanical strength (p < 0.01). Conclusions: ß-cell microencapsulation using 1% PLO, 2.5% PAA, 1.8% SA, 4.5% USG and the bile acid UDCA (4%) has good potential in cell transplantation and diabetes treatment.

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