Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
MetadataShow full item record
Resistance to the Australian pea aphid (PA; Acyrthosiphon pisum) biotype in cultivar Jester of the model legume Medicago truncatula is mediated by a single dominant gene and is phloem-mediated. The genetic map position for this resistance gene, APR (Acyrthosiphon pisum resistance), is provided and shows that APR maps 39 centiMorgans (cM) distal of the A. kondoi resistance (AKR) locus, which mediates resistance to a closely related species of the same genus bluegreen aphid (A. kondoi). The APR region on chromosome 3 is dense in classical nucleotide binding site leucine-rich repeats (NLRs) and overlaps with the region harbouring the RAP1 gene which confers resistance to a European PA biotype in the accession Jemalong A17. Further screening of a core collection of M. truncatula accessions identified seven lines with strong resistance to PA. Allelism experiments showed that the single dominant resistance to PA in M. truncatula accessions SA10481 and SA1516 are allelic to SA10733, the donor of the APR locus in cultivar Jester. While it remains unclear whether there are multiple PA resistance genes in an R-gene cluster or the resistance loci identified in the other M. truncatula accessions are allelic to APR, the introgression of APR into current M. truncatulacultivars will provide more durable resistance to PA.
This open access article is distributed under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Showing items related by title, author, creator and subject.
Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatulaKamphuis, L.; Gao, L.; Singh, Karambir (2012)Background: Cowpea aphid (CPA; Aphis craccivora) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. ...
Phoma medicaginis stimulates the induction of the octadecanoid and phenylpropanoid pathways in Medicago truncatulaKamphuis, L.; Williams, A.; Kuster, H.; Trengove, R.; Singh, K.; Oliver, R.; Ellwood, Simon (2012)Gene expression changes and metabolite abundances were measured during the interaction of Medicago truncatula with the fungal necrotrophic pathogen Phoma medicaginis in leaf tissue of susceptible and resistant accessions. ...
Identification of sources of resistance to Phoma medicaginis isolates in Medicago truncatula SARDI core collection accessions, and multigene differentiation of isolatesEllwood, Simon; Kamphuis, L.; Oliver, Richard (2006)Phoma medicaginis is a necrotrophic fungal pathogen, commonly found infecting the annual medic Medicago truncatula. To differentiate eight P. medicaginis isolates, five gene regions were examined: actin, β-tubulin, ...