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dc.contributor.authorGhassemifar, Reza
dc.contributor.authorRedmond, S.
dc.contributor.authorZainuddin
dc.contributor.authorChirila, T.
dc.date.accessioned2017-01-30T13:43:51Z
dc.date.available2017-01-30T13:43:51Z
dc.date.created2016-09-12T08:36:53Z
dc.date.issued2010
dc.identifier.citationGhassemifar, R. and Redmond, S. and Zainuddin and Chirila, T. 2010. Advancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes. Journal of Biomaterials Applications. 24 (7): pp. 591-606.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/34502
dc.identifier.doi10.1177/0885328209104289
dc.description.abstract

Human tympanic membrane cells (hTMCs), harvested from tympanic membrane (TM) explants, were grown in culture and then seeded on membranes prepared from silkworm (Bombyx mori) silk fibroin (BMSF) and on tissue-culture plastic membranes (PET). Fibroin was isolated from silk cast into membranes with a thickness of 10-15 µm. The hTMCs were cultured on both materials for 15 days in a serum-containing culture medium. The cells grown on both substrata were subjected to nuclear staining (DAPI) and counted. Further, the cultures were immunostained for a number of protein markers related to the epithelial/keratinocyte phenotype and cell adhesion complexes. The BMSF membranes supported levels of hTMC growth higher than that observed on the PET membranes. The immunofluorochemical analysis indicated unequivocally that BMSF is a more suitable substratum than PET with respect to the growth patterns, proliferation, and cell-cell contact and adhesion. BMSF appear as a promising substratum in the tissue-engineered constructs for the replacement of TM in case of nonhealing perforations.

dc.publisherSage Publications
dc.titleAdvancing towards a tissue-engineered tympanic membrane: Silk fibroin as a substratum for growing human eardrum keratinocytes
dc.typeJournal Article
dcterms.source.volume24
dcterms.source.number7
dcterms.source.startPage591
dcterms.source.endPage606
dcterms.source.issn0885-3282
dcterms.source.titleJournal of Biomaterials Applications
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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