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Isolation and characterization of microsatellite markers for the banded ironstone endemic acacia karina (Leguminosae: Mimosaceae) and cross-species amplification with A. stanleyi and A. jibberdingensis

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Nevill, Paul
Anthony, J.
Krauss, S.
Date
2010
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Journal Article

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Nevill, P. and Anthony, J. and Krauss, S. 2010. Isolation and characterization of microsatellite markers for the banded ironstone endemic acacia karina (Leguminosae: Mimosaceae) and cross-species amplification with A. stanleyi and A. jibberdingensis. Conservation Genetics Resources. 2 (SUPPL.1): pp. 321-323.
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Conservation Genetics Resources
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Department of Environment and Agriculture
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Abstract

Microsatellite markers were developed for the banded ironstone endemic shrub Acacia karina to examine genetic diversity, range-wide differentiation and mating system parameters. Nine loci were developed and in a sample of 20 individuals from one population the number of alleles ranged from 4 to 12 per locus and observed heterozygosities from 0.556 to 0.824. All loci were tested for cross-species amplification in two other south-west Australian Acacia species thought to be closely related to A. karina. Of these nine loci, eight were polymorphic in A. jibberdingensis and three in A. stanleyi. © Springer Science+Business Media B.V. 2010.