Characterization of cellulose production by a Gluconacetobacter xylinus strain from Kombucha
MetadataShow full item record
The aims of this work were to characterize and improve cellulose production by a Gluconoacetobacter xylinus strain isolated from Kombucha and determine the purity and some structural features of the cellulose from this strain. Cellulose yield in tea medium with both black tea and green tea and in Hestrin and Schramm (HS) medium under both static and agitated cultures was compared. In the tea medium, the highest cellulose yield was obtained with green tea (~0.20 g/L) rather than black tea (~0.14 g/L). Yield in HS was higher (~0.28 g/L) but did not differ between static and agitated incubation. 1H-NMR and 13C-NMR spectroscopy indicated that the cellulose is pure (free of acetan) and has high crystallinity, respectively. Cellulose yield was improved by changing the type and level of carbon and nitrogen source in the HS medium. A high yield of ~2.64 g/L was obtained with mannitol at 20 g/L and corn steep liquor at 40 g/L in combination. In the tea medium, tea at a level of 3 g/L gave the highest cellulose yield and the addition of 3 g/L of tea to the HS medium increased cellulose yield to 3.34 g/L. In conclusion, the G. xylinus strain from Kombucha had different cellulose-producing characteristics than previous strains isolated from fruit. Cellulose was produced in a pure form and showed high potential applicability. Our studies extensively characterized cellulose production from a G. xylinus strain from Kombucha for the first time, indicating both similarities and differences to strains from different sources. © 2008 Springer Science+Business Media, LLC.
Showing items related by title, author, creator and subject.
Formation and characteristics of glucose oligomers during the hydrolysis of cellulose in hot-compressed waterYu, Yun (2009)Energy production from fossil fuels results in significant carbon dioxide emission, which is a key contributor to global warming and the problems related to climate change. Biomass is recognized as an important part of ...
Influence of different carbon sources on bacterial cellulose production by Gluconacetobacter xylinus strain ATCC 53524Mikkelsen, D.; Flanagan, B.; Dykes, Gary; Gidley, M. (2009)Aims: To determine the effect of carbon sources on cellulose produced by Gluconacetobacter xylinus strain ATCC 53524, and to characterize the purity and structural features of the cellulose produced. Methods and Results: ...
Differences in Water-Soluble Intermediates from Slow Pyrolysis of Amorphous and Crystalline CelluloseLiu, Dawei; Yu, Yun; Wu, Hongwei (2013)The paper reports the significant differences in the pyrolysis behavior between amorphous and crystalline cellulose. The strong hydrogen bonding networks in crystalline cellulose appear to preserve the sugar ring structure ...