Cell-autonomous complementation of mlo resistance using a biolistic transient expression system
dc.contributor.author | Shirasu, K. | |
dc.contributor.author | Nielsen, K. | |
dc.contributor.author | Piffanelli, P. | |
dc.contributor.author | Oliver, Richard | |
dc.contributor.author | Schulze-Lefert, P. | |
dc.date.accessioned | 2017-01-30T13:51:57Z | |
dc.date.available | 2017-01-30T13:51:57Z | |
dc.date.created | 2010-11-15T01:18:43Z | |
dc.date.issued | 1999 | |
dc.identifier.citation | SHIRASU K, NIELSEN K, PIFFANELLI P, OLIVER R & SCHULZE-LEFERT P (1999) Cell-autonomous complementation of mlo resistance using a biolistic transient expression system. Plant Journal 17 293-299 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/35831 | |
dc.identifier.doi | 10.1046/j.1365-313X.1999.00376.x | |
dc.description.abstract |
The barley geneMloencodes a prototype of a novel class of plant proteins. Inmlomutants, absence of the 60 kDa wild-type Mlo protein results in broad-spectrum resistance to the powdery mildew fungus,Erysiphe graminisf. sp.hordei. To directly assess its function,Mlowas transiently expressed with a marker gene encoding a modified green fluorescent protein (GFP) in leaf epidermal cells ofmloresistant barley lines. Fungal inoculation of epidermal cells transfected with wild-typeMloled to haustorium formation and abundant sporulation. Therefore, expression of the wild-typeMlogene, inmloresistant genotypes, is both necessary and sufficient to restore susceptibility to fungal attack. Complementation ofmloresistance alleles was restricted to single host cells, indicating a cell-autonomous function for the wild-type Mlo protein. We discuss our findings with respect to source–sink relationships of plants and biotrophic fungi and the potentially wide-ranging use of the transient complementation assay to analyse host compatibility and defence in response to powdery mildew attack. | |
dc.title | Cell-autonomous complementation of mlo resistance using a biolistic transient expression system | |
dc.type | Journal Article | |
curtin.note |
A copy of this item may be available from Professor Richard Oliver | |
curtin.note |
Email: Richard.oliver@curtin.edu.au | |
curtin.accessStatus | Fulltext not available | |
curtin.faculty | Department of Environmental & Agriculture | |
curtin.faculty | School of Agriculture and Environment | |
curtin.faculty | Faculty of Science and Engineering |