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    An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus

    Access Status
    Fulltext not available
    Authors
    Waryah, Charlene Babra
    Gogoi-Tiwari, Jully
    Wells, Kelsi
    Mukkur, Trilochan
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Waryah, C.B. and Gogoi-Tiwari, J. and Wells, K. and Mukkur, T. 2016. An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus. Folia Microbiologica. 61 (6): pp. 473-478.
    Source Title
    Folia Microbiologica
    DOI
    10.1007/s12223-016-0459-6
    ISSN
    0015-5632
    School
    School of Biomedical Sciences
    Remarks

    © 2016 Institute of Microbiology, Academy of Sciences of the Czech Republic

    URI
    http://hdl.handle.net/20.500.11937/38024
    Collection
    • Curtin Research Publications
    Abstract

    Attachment of bacterial pathogens to the niche tissue in the host is the first step in biofilm formation leading to colonization and establishment of infection in the host. While the most common method used for determining the potential role of a bacterial antigen in biofilm formation has been demonstration of loss of this property using specific knockout mutants, it is an expensive and a laborious procedure. This study describes an alternative immunological assay for identification of attachment antigens of Staphylococcus aureus, potentially important in the development of an effective vaccine against infections caused by this pathogen. The method is based upon the concept of inhibition of attachment of S. aureus to PEGs coated with virulence antigen-specific antibodies. Antibodies used for validation of this assay were specific for ClfA, FnBPA, SdrD, PNAG and a-toxin, accredited biofilm-associated antigens of S. aureus.

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