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dc.contributor.authorCarnagarin, R.
dc.contributor.authorCarlessi, R.
dc.contributor.authorNewsholme, P.
dc.contributor.authorDharmarajan, Arunasalam
dc.contributor.authorDass, C.
dc.date.accessioned2017-01-30T14:10:58Z
dc.date.available2017-01-30T14:10:58Z
dc.date.created2016-08-02T19:30:17Z
dc.date.issued2016
dc.identifier.citationCarnagarin, R. and Carlessi, R. and Newsholme, P. and Dharmarajan, A. and Dass, C. 2016. Pigment epithelium-derived factor stimulates skeletal muscle glycolytic activity through NADPH oxidase-dependent reactive oxygen species production. International Journal of Biochemistry and Cell Biology. 78: pp. 229-236.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/38035
dc.identifier.doi10.1016/j.biocel.2016.06.013
dc.description.abstract

Pigment epithelium-derived factor is a multifunctional serpin implicated in insulin resistance in metabolic disorders. Recent evidence suggests that exposure of peripheral tissues such as skeletal muscle to PEDF has profound metabolic consequences with predisposition towards chronic conditions such as obesity, type 2 diabetes, metabolic syndrome and polycystic ovarian syndrome. Chronic inflammation shifts muscle metabolism towards increased glycolysis and decreased oxidative metabolism. In the present study, we demonstrate a novel effect of PEDF on cellular metabolism in mouse cell line (C2C12) and human primary skeletal muscle cells. PEDF addition to skeletal muscle cells induced enhanced phospholipase A2 activity. This was accompanied with increased production of reactive oxygen species in a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent manner that triggered a shift towards a more glycolytic phenotype. Extracellular flux analysis and glucose consumption assays demonstrated that PEDF treatment resulted in enhanced glycolysis but did not change mitochondrial respiration. Our results demonstrate that skeletal muscle cells express a PEDF-inducible oxidant generating system that enhances glycolysis but is sensitive to antioxidants and NADPH oxidase inhibition.

dc.publisherPergamon
dc.titlePigment epithelium-derived factor stimulates skeletal muscle glycolytic activity through NADPH oxidase-dependent reactive oxygen species production
dc.typeJournal Article
dcterms.source.volume78
dcterms.source.startPage229
dcterms.source.endPage236
dcterms.source.issn1357-2725
dcterms.source.titleInternational Journal of Biochemistry and Cell Biology
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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