Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator
|dc.identifier.citation||Huu, N. and Ryder, W. and Zeps, N. and Flasza, M. and Chiu, M. and Hanby, A. and Poulsom, R. et al. 2008. Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator. Journal of Pathology. 216 (1): pp. 93-102.|
WWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epithelial cells and demonstrated increased cell growth and anchorage- independent colony formation. RNAi knockdown of WWP1 expression in T47D and MCF7 breast tumour cell lines reduced anchorage-independent colony formation. We used WWP1 protein expression levels, in combination with its subcellular localization, to classify breast tumours into four categories. Surprisingly, a category with low/absent WWP1 expression displayed a consistently worse prognosis compared with WWPl-expressing tumours. Importantly, the association with disease-free survival was independent of the status of other commonly used prognostic indicators. Thus, WWP1 is a prognostic marker and may be a potential therapeutic target for a subset of breast tumours. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
|dc.publisher||John Wiley & Sons Ltd|
|dc.title||Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator|
|dcterms.source.title||Journal of Pathology|
|curtin.accessStatus||Fulltext not available|
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