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dc.contributor.authorHuu, N.
dc.contributor.authorRyder, W.
dc.contributor.authorZeps, Nikolajs
dc.contributor.authorFlasza, M.
dc.contributor.authorChiu, M.
dc.contributor.authorHanby, A.
dc.contributor.authorPoulsom, R.
dc.contributor.authorClarke, R.
dc.contributor.authorBaron, M.
dc.identifier.citationHuu, N. and Ryder, W. and Zeps, N. and Flasza, M. and Chiu, M. and Hanby, A. and Poulsom, R. et al. 2008. Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator. Journal of Pathology. 216 (1): pp. 93-102.

WWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epithelial cells and demonstrated increased cell growth and anchorage- independent colony formation. RNAi knockdown of WWP1 expression in T47D and MCF7 breast tumour cell lines reduced anchorage-independent colony formation. We used WWP1 protein expression levels, in combination with its subcellular localization, to classify breast tumours into four categories. Surprisingly, a category with low/absent WWP1 expression displayed a consistently worse prognosis compared with WWPl-expressing tumours. Importantly, the association with disease-free survival was independent of the status of other commonly used prognostic indicators. Thus, WWP1 is a prognostic marker and may be a potential therapeutic target for a subset of breast tumours. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

dc.publisherJohn Wiley & Sons Ltd
dc.titleTumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator
dc.typeJournal Article
dcterms.source.titleJournal of Pathology
curtin.accessStatusFulltext not available

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